PALM and STORM are often used as synonyms, and in fact they have a lot in common. But there are slight differences that can be important for your application. And then there are other superresolution techniques, too – like STED and MINFLUX. Details >
Knowledge Base
Have you ever wondered how superresolution microscopy works? What’s the difference between STED, STORM, and MINFLUX? What is “resolution” and what is a “PSF”? What is so special about the STEDYCON? Read on to find out.
If you have any suggestions, questions or ideas for our knowledge base, we would be very happy to hear from you.
ContactEverything about microscopes, dyes, and superresolution
All
#2photon
#aberrationcorrection
#adaptiveillumination
#adaptiveoptics
#antibody
#arraydetection
#basicprinciples
#biology
#comparison
#confocal
#deformablemirror
#diffractionlimit
#donut
#expansion-technique
#fluorescence
#immunofluorescence
#labeling
#laser
#lightmicroscopy
#livingcells
#MATRIX
#MINFLUX
#modules
#nanobody
#nanometer
#optics
#PAINT
#PALM
#resolution
#selflabelingproteins
#SMLM
#STED
#STEDYCON
#STORM
#superresolution
#TEM
#tracking
#virology
#widefield
Which microscope has the best resolution?
The elctron microscope achieves the highest magnification and resolution. But does "highest" always equal "best"? Well, that depends on what you want to do with the resolution. Details >
Today’s research microscopes are increasingly powerful, modular, and combinatorial. There’s a lot of options out there. While the price is unquestionably a deal-breaker for purchase, a more helpful criterion is value. Details >
Today’s high-end fluorescence microscopy is unthinkable without lasers. Reason enough to take a closer look at these sophisticated light sources. Details >
MINFLUX reaches unprecedented spatio-temporal resolution in light microscopy and provides 2D and 3D localization precisions in the single-digit nanometer range. Details >
Superresolution for biology: when size, time, and context matter
The spatial resolution achievable with today’s light microscopes has unveiled life at the scale of individual molecules. Size is no longer a barrier to seeing biology at the most fundamental level. But life is not static. It emerges from movement and change. How do superresolution technologies hold up to the challenges of documenting dynamic biological mechanisms? Details >