2D MINFLUX nanoscopy of Y. enterocolitica sorting platform protein Halo-YscL.

Sample from Alexander Carsten, Prof. Martin Aepfelbacher, Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Germany

STED-PAINT imaging of bacterial membranes (magenta) and DNA (green) using a high concentration of the exchangeable labels.

3D MINFLUX nanoscopy of Y. enterocolitica sorting platform protein Halo-YscL in xy view of two bacteria. The z-axis is color coded.

Sample from Alexander Carsten, Prof. Martin Aepfelbacher, Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Germany

Movement of fluorophore-labeled 30S ribosomes (colored) in two E. coli bacteria (grayscale) tracked with MINFLUX.

abberior STAR RED was coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch and used to label tubulin in fixed mammalian cells via indirect immunofluorescence. The STED image was acquired with the STEDYCON system.

Proteins of the nuclear pore complex and the golgi apparatus were stained by indirect immunofluorescence using abberior STAR RED (nuclear pore complex, magenta) and abberior STAR 580 (golgi, green) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.

Vimentin and tubulin were stained by indirect immunofluorescence using abberior STAR RED (vimentin, orange) and abberior STAR 580 (tubulin, cyan) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.

Indirect immunofluorescence staining with abberior STAR dyes coupled to polyclonal anti-mouse secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch shows excellent results in 3-color STED imaging. Staining was performed according to the premix & stain protocol. Vimentin (abberior STAR RED, green), tubulin (abberior STAR 580, magenta) and dsDNA (abberior STAR 460L, blue) were labeled in fixed mammalian cells and visualized with the STEDYCON.

OMP25-SNAP protein in mitochondria labeled with abberior LIVE 610 SNAP (magenta) and Actin^K118TAG labeled with abberior LIVE 550 click (green). Movie with 60 frames.

2-color STED image of a living cell expressing actin K118TAG incorporating TCO*A labeled with abberior LIVE 590 click (green). Plasma membrane is highlighted with abberior STAR RED membrane (magenta).

2-color STED image of a living cell expressing actin^K118TAG incorporating TCO*A labeled with abberior LIVE 550 click (cyan). Tubulin filaments were stained with our direct probe abberior LIVE 610 tubulin (yellow).

Perfect 3-color combination for STED @ 775 nm. abberior STAR RED NUP98 (red) and STAR ORANGE vimentin (cyan) complemented by the new STAR 460L tubulin (yellow). Cultured mammalian cells were fixed and labeled by indirect immunofluorescence.

We are happy to introduce the first commercial long Stokes-shift dye for live-cell imaging applications. The NEW abberior LIVE 460L makes 3-color live-cell STED at 775 nm possible!

Live-cell STED image of a cell expressing a SNAP-tag® in an outer mitochondrial membrane protein visualized by our abberior LIVE 460L SNAP (orange). Tubulin filaments are highlighted with LIVE 610 (cyan) and the DNA with LIVE 560 DNA (red).

STED time-lapse of living mammalian cell stained with abberior STAR ORANGE membrane.

Two color live-cell STED and confocal image of a mammalian cultured cell stained with abberior STAR RED membrane (orange) and abberior LIVE 590 actin (cyan).

Comparison of STED and confocal of a living mammalian cell stained with abberior STAR ORANGE membrane.