Two color live-cell STED image of mitochondria in a mammalian cell expressing a SNAP-tag® OMP25 fusion protein. The Cristae sturctures are stained with our abberior LIVE ORANGE mito probe (green) while OMP25 is visualized by our abberior LIVE 610 SNAP probe (magenta). The image was acquired with a STEDYCON microscope.

The SNAP-OMP25 plasmid was a gift from Francesca Bottanelli, FU Berlin.

Composite two-color live cell STED image of an adherent mammalian cell. The cristae of the mitochondria in this living cell were directly labelled with abberior LIVE ORANGE mito (green), while the microtubules were stained with LIVE 610 tubulin (magenta). This image was acquired with a FACILITY microscope.

Composite two-color live cell STED image of an adherent mammalian cell. The cristae of the mitochondria in this living cell were directly labelled with abberior LIVE ORANGE mito (yellow), while the microtubules were stained with LIVE 610 tubulin (blue). This image was acquired with a FACILITY microscope.

OMP25-SNAP protein in mitochondria labeled with abberior LIVE 610 SNAP (magenta) and Actin^K118TAG labeled with abberior LIVE 550 click (green). Movie with 60 frames.

2-color STED image of a living cell expressing actin K118TAG incorporating TCO*A labeled with abberior LIVE 590 click (green). Plasma membrane is highlighted with abberior STAR RED membrane (magenta).

2-color STED image of a living cell expressing actin^K118TAG incorporating TCO*A labeled with abberior LIVE 550 click (cyan). Tubulin filaments were stained with our direct probe abberior LIVE 610 tubulin (yellow).

Perfect 3-color combination for STED @ 775 nm. abberior STAR RED NUP98 (red) and STAR ORANGE vimentin (cyan) complemented by the new STAR 460L tubulin (yellow). Cultured mammalian cells were fixed and labeled by indirect immunofluorescence.

We are happy to introduce the first commercial long Stokes-shift dye for live-cell imaging applications. The NEW abberior LIVE 460L makes 3-color live-cell STED at 775 nm possible!

Live-cell STED image of a cell expressing a SNAP-tag® in an outer mitochondrial membrane protein visualized by our abberior LIVE 460L SNAP (orange). Tubulin filaments are highlighted with LIVE 610 (cyan) and the DNA with LIVE 560 DNA (red).

STED time-lapse of living mammalian cell stained with abberior STAR ORANGE membrane.

Two color live-cell STED and confocal image of a mammalian cultured cell stained with abberior STAR RED membrane (orange) and abberior LIVE 590 actin (cyan).

Comparison of STED and confocal of a living mammalian cell stained with abberior STAR ORANGE membrane.

Comparison of STED and confocal of a living mammalian cell stained with abberior STAR 488 membrane. Images were acquired with our FACILITY and STED @ 595 nm.

Confocal time-lapse of living mammalian cell stained with abberior STAR 488 membrane.

abberior CAGE 552 is a caged dye which is initially nonfluorescent and colorless substance which, upon photolysis with UV light, readily transforms irreversibly into a red and highly fluorescent dye.

The cytoskeleton is visible after UV induced uncaging of abberior CAGE 552.

3D MINFLUX image of the mitochondrial import receptor Tom20 labeled with abberior FLUX 647 in fixed mammalian cells using indirect immunolabeling. Please note that Tom20 is only localized at the mitochondrial surface. The axial coordinate is color-coded.