Sample gallery
Fluorescence imaging, whether at confocal, STED or MINFLUX resolution, guarantees unique insights into the function and structure of life at the molecular level. Besides the scientific information content, some sample portraits provide simply beautiful images. Enjoy browsing our sample gallery.
the fine art of science
Description
Paraffin section of gut biopsy stained for Ki67 (abberior STAR ORANGE), Muc2 (abberior STAR RED), and DAPI.
Modules:
Description
Gain in both signal-to-background ratio and resolution: MATRIX detection dramatically improves a conventional STED image of the zebrafish olfactory epithelium, resulting in a perfect and crystal-clear image revealing even more detail than STED alone can.
Modules:
Description
xz section of a stage 17 Drosophila embryo stained for chitin (abberior LIVE 610, green) and DNA (abberior LIVE 550, cyan).
RAYSHAPE preserves resolution and brightness over the whole sample depth of about 200 µm by dynamically redirecting aberrated light to the right places.
In comparison, mechanical optics using a correction collar can only correct a limited z-range of approximately 20 µm
Modules:
Description
Drosophila stage 12 embryo, imaged with RAYSHAPE, stained for tubulin with abberior STAR RED and for DNA with abberior LIVE 550.
Modules:
Description
Deep tissue imaging with RAYSHAPE of a stage 17 Drosophila embryo stained for chitin with abberior LIVE 610.
Modules:
Description
Clear and detailed 3D rendering of trachea imaged with RAYSHAPE and MATRIX STED. Chitin was stained with abberior LIVE 610.
Modules:
Description
3D STED xz section of a Drosophila embryo trachea, imaged with RAYSHAPE and without abberation correction, depth 15 µm. Chitin was stained with abberior LIVE 610.
Modules:
Description
2D MINFLUX nanoscopy of Y. enterocolitica sorting platform protein Halo-YscL.
Sample from Alexander Carsten, Prof. Martin Aepfelbacher, Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Germany
Description
STED-PAINT imaging of bacterial membranes (magenta) and DNA (green) using a high concentration of the exchangeable labels.
Description
3D MINFLUX nanoscopy of Y. enterocolitica sorting platform protein Halo-YscL in xy view of two bacteria. The z-axis is color coded.
Sample from Alexander Carsten, Prof. Martin Aepfelbacher, Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Germany
Description
abberior STAR RED was coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch and used to label tubulin in fixed mammalian cells via indirect immunofluorescence. The STED image was acquired with the STEDYCON system.
Description
Proteins of the nuclear pore complex and the golgi apparatus were stained by indirect immunofluorescence using abberior STAR RED (nuclear pore complex, magenta) and abberior STAR 580 (golgi, green) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.
Description
Vimentin and tubulin were stained by indirect immunofluorescence using abberior STAR RED (vimentin, orange) and abberior STAR 580 (tubulin, cyan) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.
Description
Indirect immunofluorescence staining with abberior STAR dyes coupled to polyclonal anti-mouse secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch shows excellent results in 3-color STED imaging. Staining was performed according to the premix & stain protocol. Vimentin (abberior STAR RED, green), tubulin (abberior STAR 580, magenta) and dsDNA (abberior STAR 460L, blue) were labeled in fixed mammalian cells and visualized with the STEDYCON.
Description
OMP25-SNAP protein in mitochondria labeled with abberior LIVE 610 SNAP (magenta) and Actin^K118TAG labeled with abberior LIVE 550 click (green). Movie with 60 frames.
Description
Paraffin section of gut biopsy stained for Ki67 (abberior STAR ORANGE), Muc2 (abberior STAR RED), and DAPI.
Modules:
Description
Gain in both signal-to-background ratio and resolution: MATRIX detection dramatically improves a conventional STED image of the zebrafish olfactory epithelium, resulting in a perfect and crystal-clear image revealing even more detail than STED alone can.
Modules:
Description
xz section of a stage 17 Drosophila embryo stained for chitin (abberior LIVE 610, green) and DNA (abberior LIVE 550, cyan).
RAYSHAPE preserves resolution and brightness over the whole sample depth of about 200 µm by dynamically redirecting aberrated light to the right places.
In comparison, mechanical optics using a correction collar can only correct a limited z-range of approximately 20 µm
Modules:
Description
Drosophila stage 12 embryo, imaged with RAYSHAPE, stained for tubulin with abberior STAR RED and for DNA with abberior LIVE 550.
Modules:
Description
Deep tissue imaging with RAYSHAPE of a stage 17 Drosophila embryo stained for chitin with abberior LIVE 610.
Modules:
Description
Clear and detailed 3D rendering of trachea imaged with RAYSHAPE and MATRIX STED. Chitin was stained with abberior LIVE 610.
Modules:
Description
3D STED xz section of a Drosophila embryo trachea, imaged with RAYSHAPE and without abberation correction, depth 15 µm. Chitin was stained with abberior LIVE 610.
Modules:
Description
2D MINFLUX nanoscopy of Y. enterocolitica sorting platform protein Halo-YscL.
Sample from Alexander Carsten, Prof. Martin Aepfelbacher, Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Germany
Description
STED-PAINT imaging of bacterial membranes (magenta) and DNA (green) using a high concentration of the exchangeable labels.
Description
3D MINFLUX nanoscopy of Y. enterocolitica sorting platform protein Halo-YscL in xy view of two bacteria. The z-axis is color coded.
Sample from Alexander Carsten, Prof. Martin Aepfelbacher, Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Germany
Description
Movement of fluorophore-labeled 30S ribosomes (colored) in two E. coli bacteria (grayscale) tracked with MINFLUX.
Description
abberior STAR RED was coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch and used to label tubulin in fixed mammalian cells via indirect immunofluorescence. The STED image was acquired with the STEDYCON system.
Description
Proteins of the nuclear pore complex and the golgi apparatus were stained by indirect immunofluorescence using abberior STAR RED (nuclear pore complex, magenta) and abberior STAR 580 (golgi, green) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.
Description
Vimentin and tubulin were stained by indirect immunofluorescence using abberior STAR RED (vimentin, orange) and abberior STAR 580 (tubulin, cyan) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.
Description
Indirect immunofluorescence staining with abberior STAR dyes coupled to polyclonal anti-mouse secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch shows excellent results in 3-color STED imaging. Staining was performed according to the premix & stain protocol. Vimentin (abberior STAR RED, green), tubulin (abberior STAR 580, magenta) and dsDNA (abberior STAR 460L, blue) were labeled in fixed mammalian cells and visualized with the STEDYCON.
Description
OMP25-SNAP protein in mitochondria labeled with abberior LIVE 610 SNAP (magenta) and Actin^K118TAG labeled with abberior LIVE 550 click (green). Movie with 60 frames.