TIMEBOW

abberior is all about breaking barriers. With TIMEBOW, we have once again succeeded. TIMEBOW is a reliable method for measuring fluorescence lifetime with and without super resolution. In combination with our highly sensitive APDs and the groundbreaking MATRIX detector, you get the best out of every aspect of the signal – as easy as 1-2-3! With TIMEBOW, your research shines in perfect color.

gives time a color

TIMEBOW at a glance >

Background-free MATRIX STED image of actin in microvilli of Caco2 cells

MATRIX Detector

Many eyes see more than one. The MATRIX detector drastically improves signal-to-background ratio, resolution, and dynamic range.

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TIMEBOW Imaging

TIMEBOW lifetime imaging for stunning results at confocal and STED super-resolution.

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Thanks to adaptive illumination fluorophore bleaching is substantially reduced

Adaptive Illumination

Brings down the light dose on your sample and lables dramatically. Key ingredient for volume and live-cell superresolution.

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Surface rendering of a z-stack of Nephrin in the kidney

Adaptive Optics

Puts every beam in perfect shape

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Axial STED image of actin in mouse inner ear hair cells

EASY3D

3D-STED and aberration control, the easy way

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Three-color STED image of microtubules, myosin and actin in the growth cone of a neuron's axon

RAINBOW Detection

Continuously variable spectral detection.

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STED Lasers

All-pulsed STED lasers for maximum resolution and minimal bleaching.

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Autoalignment

Every beam in order, including pinhole and STED-shape

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Autofocus

Continuous focus-lock, even with STED

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Excitation Lasers

Pulsed excitation lasers for virtually every wavelength.

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Accessories

Things to make your life easier

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Custom upright 3D STED microscope by abberior

Custom Solutions

We offer solutions for even the most challenging applications. Everything that can be done, we will do.

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FAQ Video 36

“TIMEBOW gives time a color!”

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FAQ Video 37

“How does TIMEBOW lifetime imaging work and why is MATRIX array detection a perfect match?”

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FAQ Video 12

 “Does abberior offer lifetime imaging?”

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TIMEBOW

lifetime data evaluation at its best

Lifetime imaging is an important method and certainly nothing new. That is why all abberior microscopes could for a long time be equipped with lifetime imaging.

But you rightly expect more from the inventors of STED and MINFLUX. More options, more speed, more ease of use, more choices. Here comes TIMEBOW. The most powerful tool for turning lifetime data into beautiful color images. The direct integration into our LIGHTBOX software allows you to work with the data seamlessly.

Intuitive LIGHTBOX software with TIMEBOW  

Picture 1
Color-coded fluorescence lifetime in an autofluorescent sample: plant stem cross section of Convallaria.

Intuitive LIGHTBOX software with TIMEBOW  

Picture 2
The grey intensity image without lifetime data. Image quality is already improved by removing the background with MATRIX detection.

Intuitive LIGHTBOX software with TIMEBOW  

Picture 3
One click opens the phasor plot and gives access to lifetime data.

Intuitive LIGHTBOX software with TIMEBOW  

Picture4
A color-coded lifetime image is just another click away.

TIMEBOW

fun at work

To give you the best way to work with lifetime data, TIMEBOW uses phasor analysis. This turns the temporal dynamics of fluorescence decay into a convenient plot which directly visualizes different lifetime populations. The plot is interactive and gives you the tools you need to process lifetime information within seconds.

Easy separation of structures thanks to TIMEBOW

Picture 1
Lifetime separated into four species in a plant stem cross section of Convallaria.

Easy separation of structures thanks to TIMEBOW

Picture 2
Image quality is improved by removing the background with MATRIX detection. TIMEBOW gives you the interactive phasor plot to work with.

Easy separation of structures thanks to TIMEBOW

Picture 3
By simply drawing circular filters in the phasor plot, different species can be defined. Starting with the longest lifetimes …

Easy separation of structures thanks to TIMEBOW

Picture 4
… and then defining more species by adding more circles …

Easy separation of structures thanks to TIMEBOW

Picture 5
… and more circles … and more species …

Easy separation of structures thanks to TIMEBOW

Picture 6
… until everything is separated.

Easy separation of structures thanks to TIMEBOW

Picture 7
Finally, these four species are combined, showing a clearly distinct distribution in the sample.

TIMEBOW

you will simply love it

Our super-resolution imaging already produces stunning images along all spatial dimensions. Now we do the same with the dimension of time. Grey images turn into colorful sample portraits as color-coded lifetime plots reveal unseen structures. Fluorophores in the same spectral channel suddenly are clearly separated, giving you more channels and more flexibility than ever before.

4 colors with one STED laser? Yes of course with TIMEBOW!

Picture 1
A super-resolved four-color image recorded with only one STED laser.

4 colors with one STED laser? Yes of course with TIMEBOW!

Picture 2
Traditionally, one of the rules of labelling was: dyes with similar excitation spectra cannot be combined in one sample. But here, this is exactly what we do!

4 colors with one STED laser? Yes of course with TIMEBOW!

Picture 3
TIMEBOW allows for straightforward separation by lifetime, simply by drawing a line in the phasor plot. Now the targets can be distinguished, due to their difference in lifetime.

4 colors with one STED laser? Yes of course with TIMEBOW!

Picture 4
All separated layers are combined to create a four-color image, which already looks stunning in confocal …

4 colors with one STED laser? Yes of course with TIMEBOW!

Picture 5
… and WOW with super-resolution.

TIMEBOW + MATRIX

a perfect match only from abberior

Of course, TIMEBOW can be combined with our MATRIX array detector. This combination is even more than the sum of its parts. It brings you: higher signal-to-background ratio, better resolution and an impressive dynamic range. Every one of the more than twenty sensor elements of the MATRIX is used to simultaneously record both lifetime information and information about the origin of photons relative to the focal plane. With this data, we get rid of background better than a confocal pinhole ever could and we render stunning images not only richer in information, but also with exceptional contrast.

TIMEBOW is not only used for lifetime measurements, but can also be used to boost STED resolution. This is because fluorophores far out from the center of the donut experience higher STED powers and hence shorter lifetimes. In other words, the lifetime encodes additional spatial information. Using it effectively increases the resolution of the image.

Sharpening with TIMEBOW

Picture 1
Actin in microvilli, with the background removed using MATRIX. STED imaging has a unique property, which TIMEBOW uses to further boost the resolution…

Sharpening with TIMEBOW

Picture 2
… by removing short-lived components that are not in the very center of the STED-donut. This is done by simply dragging the separation line in the phasor plot.
Sample by Prof. Dorothee Günzel and Jörg Piontek (Charité).

Lifetime imaging

For each individual photon of the fluorescence signal we can measure not only where it came from, but also when. Since the latter depends on what type of molecule it is and also on its environment, there is tons of valuable information waiting for us. For example, we can use temporal dynamics to distinguish different fluorophores, different structures labeled with the same fluorophore, and we can even use it to boost resolution.

So what’s the catch? Well, in the quantum world all this information is hidden behind a layer of chance. Photons are simply not as predictable as we would like them to be, so we have to use probability and statistics to extract the information we want. Fortunately then, TIMEBOW takes care of all of this and we made it super easy and intuitive, so it fits seamlessly into your lab routine. And together with MATRIX it’s a winning combination.

Array detection

Seeing things from different viewpoints broadens your perspective. For example, we all know that three-dimensional human vision is a product of us having two eyes instead of one, the simplest form of an array.

With our MATRIX, we use more than twenty individual detectors to physically distinguish between out-of-focus signal and photons coming right from the focal plane. Just like your eyes know what’s close and what’s far away.

This allows us to remove background much better than a mechanical pinhole could, and it significantly improves contrast, especially in thick samples. And together with TIMEBOW it’s a winning combination, too.

  • Turn temporal dynamics into color-coded lifetime images
  • Super-resolved four-color images with only one STED laser
  • Boost confocal and STED resolution
  • Together with MATRIX: higher signal-to-background ratio, better resolution and impressive dynamic range – only available from abberior

TIMEBOW lifetime imaging for stunning results with confocal and STED super-resolution

Background-free MATRIX STED image of actin in microvilli of Caco2 cells

MATRIX Detector

Many eyes see more than one. The MATRIX detector drastically improves signal-to-background ratio, resolution, and dynamic range.

Details >

Thanks to adaptive illumination fluorophore bleaching is substantially reduced

Adaptive Illumination

Brings down the light dose on your sample and lables dramatically. Key ingredient for volume and live-cell superresolution.

Details >

Emblem of the Werner-von-Siemens-Ring foundation

Stefan Hell and Heads of BioNTech receive Werner-von-Siemens Ring 2022

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