Indirect immunofluorescence staining with abberior STAR dyes coupled to polyclonal anti-mouse secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch shows excellent results in 3-color STED imaging. Staining was performed according to the premix & stain protocol. Vimentin (abberior STAR RED, green), tubulin (abberior STAR 580, magenta) and dsDNA (abberior STAR 460L, blue) were labeled in fixed mammalian cells and visualized with the STEDYCON.

Proteins of the nuclear pore complex and the golgi apparatus were stained by indirect immunofluorescence using abberior STAR RED (nuclear pore complex, magenta) and abberior STAR 580 (golgi, green) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.

Vimentin and tubulin were stained by indirect immunofluorescence using abberior STAR RED (vimentin, orange) and abberior STAR 580 (tubulin, cyan) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.

abberior STAR RED was coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch and used to label tubulin in fixed mammalian cells via indirect immunofluorescence. The STED image was acquired with the STEDYCON system.

Drosophila female reproductive system stained for F-actin (red) with abberior STAR RED phalloidin. abberior STAR ORANGE is highlighting a nuclear pore protein (gray).

Image was acquired with the STEDYCON tiling feature and assembled with the SVI Huygens Stitcher.

Two color live-cell STED and confocal image of a mammalian cultured cell stained with abberior STAR RED membrane (orange) and abberior LIVE 590 actin (cyan).

Comparison of STED and confocal of a living mammalian cell stained with abberior STAR 488 membrane. Images were acquired with our FACILITY and STED @ 595 nm.

Confocal time-lapse of living mammalian cell stained with abberior STAR 488 membrane.

STED time-lapse of living mammalian cell stained with abberior STAR ORANGE membrane.

Perfect 3-color combination for STED @ 775 nm. abberior STAR RED NUP98 (red) and STAR ORANGE vimentin (cyan) complemented by the new STAR 460L tubulin (yellow). Cultured mammalian cells were fixed and labeled by indirect immunofluorescence.

Four color confocal image of mammalian cultured cells. Peroxisomes were immunostained with abberior STAR RED (magenta) and nuclear pore proteins with abberior STAR 580 (green). F-actin fibers were highlighted with abberior STAR 488 phalloidin (gray).

The nucleus was visualized with DAPI (blue).

Three color confocal image of human fibroblast immunostained with abberior STAR 580 for vimentin (green) and with abberior STAR RED for tubulin (red).

DAPI was added to highlight the nucleus (blue).

Three color STED and confocal image of a mammalian cultured cell immunostained for a nuclear pore protein in green and two golgi apparatus markers in red and blue.

abberior STAR RED highlights the golgi apparatus protein giantin (red) and abberior STAR ORANGE visualizes the cis-golgi protein GM130 (blue). NUP98 proteins were stained with abberior STAR GREEN (green).

Drosophila male accessory gland stained for F-actin using abberior STAR 580 phalloidin.

Sample was prepared in cooperation with Dr. H. R. Shcherbata at MPl for Biophysical Chemistry, Göttingen, Germany.

Cultured mammalian cell immunostained for an inner and outer mitochondrial membrane marker. Outer membrane is highlighted with abberior STAR RED (red) and the inner membrane with abberior STAR ORANGE (gray).

STED and confocal images were acquired with abberior’s FACILITY microscope.