RAINBOW Detection

The abberior RAINBOW Detection pairs high transmission and quantum efficiency with optimal wavelength discrimination. There are no more compromises: you benefit from both maximum signal and full spectral flexibility! The superior detection efficiency of APDs compared to any other photon detector, including PMTs and hybrid detectors, guarantees that you collect as many precious fluorescence photons from your sample as possible. 

full contrast, full flexibility

Background-free MATRIX STED image of actin in microvilli of Caco2 cells

MATRIX Detector

Many eyes see more than one. The MATRIX detector drastically improves signal-to-background ratio, resolution, and dynamic range.

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TIMEBOW Imaging

TIMEBOW lifetime imaging for stunning results at confocal and STED super-resolution.

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Neurons stained for spectrin and adducine

FLEXPOSURE Illumination

Brings down the light dose on your sample and lables dramatically. Key ingredient for volume and live-cell superresolution.

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xz section of a stage 17 Drosophila embryo, stained for chitin and DNA

RAYSHAPE Mirror

Dynamic aberration correction with a deformable mirror over about 200 µm z-range. 140 digital actuators adjust the mirror surface within milliseconds.

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Meiotic cell of Chinese spring wheat stained for two synaptonemal complex components. TRUESHARP STED image.

TRUESHARP Deconvolution

TRUESHARP image boosting is next level deconvolution.

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Axial STED image of actin in mouse inner ear hair cells

EASY3D

3D-STED and aberration control, the easy way

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Three-color STED image of microtubules, myosin and actin in the growth cone of a neuron's axon

RAINBOW Detection

Continuously variable spectral detection.

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STED Lasers

All-pulsed STED lasers for maximum resolution and minimal bleaching.

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Autoalignment

Every beam in order, including pinhole and STED-shape

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Autofocus

Continuous focus-lock, even with STED

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Excitation Lasers

Pulsed excitation lasers for virtually every wavelength.

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Accessories

Things to make your life easier

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Custom upright 3D STED microscope by abberior

Custom Solutions

We offer solutions for even the most challenging applications. Everything that can be done, we will do.

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FAQ Video 07

“What makes the abberior RAINBOW Spectral Detection superior?”

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FAQ Video 02

“Why do we usually recommend APDs in our microscopes and why aren’t we worried about the supposedly lower dynamic range?”

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Spectral selection

with ultra-high transmission

A pair of gradient-coated filters are translated within the RAINBOW Detection in order to freely define the edges of the detection window between 400 and 800 nm. Up to four RAINBOW devices can be installed or upgraded at any time and the fluorescence can be freely distributed between every detector.

Super-sensitive

detection

Our standard detectors are avalanche photo detectors (APDs), because they have superior quantum efficiencies, up to a factor of two above hybrid detectors. This means that even when the signal levels are low, our APDs still collect plenty of photons for a meaningful image. Typical applications are superresolution STED imaging and experiments with low labeling densities designed to maintain physiological conditions. At the same time, high-signal samples are perfectly taken care of by DYNAMIC PLUS, our dead-time compensation that yields crisp images of brightly labeled samples. This way, confocal imaging is highly improved, too. Of course, you can opt between quantitative analysis of raw data or mathematical post-processing, e.g. deconvolution. Because we always give you genuine photon counts, you remain in full control of your data interpretation. Always.

Other detectors are available on request.

Sources:
1) “Leica HyD for Confocal Imaging”, Order no.: English 1593003013 ∙ 06/2015/STO, Leica Microsystems
2) “SPCM-AQRH Single Photon Counting Module”, Rev 2018-12, Excelitas Technologies Corp.

  • Gap-free spectral detection
  • Choose any wavelength band between 400 and 800 nm
  • APDs for the highest sensitivity (up to 65% quantum efficiency)
  • Free selection of excitation and STED wavelength combinations

Because every photon counts

Dr. Andreas Schönle. Caption: Physicist, Co-Founder and passionate Photon Hunter

Why do we usually recommend APDs in our microscopes and why aren’t we worried about the supposedly lower dynamic range?

Having too many photons is never a problem. Therefore, detectors with the highest quantum efficiency are always the best choice, such as in a MATRIX array.

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Axial STED image of actin in mouse inner ear hair cells

EASY3D

3D-STED and aberration control, the easy way

Details >