What has to be inside a STED microscope to achieve superresolution? How does its hardware differ from a confocal setup? (Hint: Not very much.) And what does that mean for the user? (Many good things.) Is handling a STED system any more complicated than using a confocal? (Not really.) Important questions – here are some in-depth answers. Details >
Knowledge Base
Have you ever wondered how superresolution microscopy works? What’s the difference between STED, STORM, and MINFLUX? What is “resolution” and what is a “PSF”? What is so special about the STEDYCON? Read on to find out.
If you have any suggestions, questions or ideas for our knowledge base, we would be very happy to hear from you.
ContactEverything about light microscopes, dyes, and imaging
All
#2photon
#aberrationcorrection
#adaptiveillumination
#adaptiveoptics
#antibody
#arraydetection
#basicprinciples
#biology
#comparison
#confocal
#deformablemirror
#diffractionlimit
#donut
#ExM
#fluorescence
#fourier
#immunofluorescence
#labeling
#laser
#lightmicroscopy
#livingcells
#MATRIX
#MINFLUX
#MIRAVA
#modules
#nanobody
#nanometer
#optics
#PAINT
#PALM
#resolution
#selflabelingproteins
#SIM
#SMLM
#STED
#STEDYCON
#STORM
#superresolution
#TEM
#tracking
#virology
#widefield
Which microscope has the best resolution?
The elctron microscope achieves the highest magnification and resolution. But does "highest" always equal "best"? Well, that depends on what you want to do with the resolution. Details >