Two-color MINFLUX revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cell labeled with indirect immunofluorescence using secondary antibodies coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.

MINFLUX imaging of βII spectrin in a primary hippocampal neuron labeled with abberior FLUX 680 by indirect immunofluorescence. Please note the periodic arrangement of spectrin along the axon.

MINFLUX image of axonal βII spectrin labeled with abberior FLUX 660 in primary hippocampal neurons. Note the periodic arrangement of spectrin along the axon, and the absence of any details in the confocal counterpart image.

2D MINFLUX nanoscopy of the nuclear pore complex subunits. Fixed mammalian cells expressing SNAP-tag® NUP96 were labeled with abberior FLUX 647 SNAP. In contrast to confocal microscopy, 2D MINFLUX allows to visualize the shape and arrangement of individual subunits of the nuclear pore complex.

2D MINFLUX imaging of the peroxisomal membrane protein PMP70 labeled with abberior FLUX 640 in fixed mammalian cells using indirect immunofluorescence.

Drosophila ovariole stained with abberior LIVE 560 DNA showing nuclei in different cell types of the egg chamber. Ovaries were dissected from adult female fruit flies and were fixed prior to staining.

Image was acquired with the STEDYCON tiling feature and assembled with the SVI Huygens Stitcher.

Drosophila spermatid tails were stained with abberior LIVE 610 tubulin. Testis were dissected from adult male fruit flies. Live cell imaging experiment was performed on a  INFINITY microscope.

Composite of three color live-cell image of an adherent mammalian cell. This living cell was directly labelled with abberior LIVE 510 actin (blue/green), LIVE 560 DNA (gray) and LIVE 610 tubulin. This image was acquired with a STEDYCON microscope.

Three color live-cell STED at 775 nm: living cell labelled with abberior LIVE 460L (ER, green), LIVE 560 tubulin (magenta) and LIVE 610 actin (red).

Confocal and STED time lapse of a living cell expressing SNAP-tag® fusion protein fusion protein in the endoplasmic reticulum lumen with SNAP and C-terminal tetrapeptide KDEL. SNAP-KDEL was labelled with abberior LIVE 610 SNAP ligand (benzylguanine).

The SNAP-KDEL plasmid was a gift from Francesca Bottanelli, FU Berlin.

Live cell time lapse of abberior LIVE 590 DNA probe in cultured mammalian cells.

The use of very low concentrations of our abberior LIVE dyes reduces toxicity and allows long-term imaging of dynamic processes.

Image was acquired with the FACILITY microscope.

Two color live-cell confocal and STED image of a mammalian cell expressing a SNAP-tag® OMP25 fusion protein decoration the outer membrane of mitochondria. OMP25 is visualized by our new abberior LIVE 610 SNAP ligand (orange). Tubulin filaments are highlighted with abberior LIVE 550 tubulin (cyan).

The SNAP-OMP25 plasmid was a gift from Francesca Bottanelli, FU Berlin.

Two color live-cell confocal and STED image of a mammalian cell expressing a SNAP-tag® OMP25 fusion protein decoration the outer membrane of mitochondria. OMP25 is visualized by our new abberior LIVE 610 SNAP ligand (orange). Tubulin filaments are highlighted with abberior LIVE 550 tubulin (cyan).

The SNAP-OMP25 plasmid was a gift from Francesca Bottanelli, FU Berlin.

Cultured mammalian cell immunostained for an inner and outer mitochondrial membrane marker. Outer membrane is highlighted with abberior STAR RED (red) and the inner membrane with abberior STAR ORANGE (gray).

STED and confocal images were acquired with abberior's FACILITY microscope.

Drosophila male accessory gland stained for F-actin using abberior STAR 580 phalloidin.

Sample was prepared in cooperation with Dr. H. R. Shcherbata at MPl for Biophysical Chemistry, Göttingen, Germany.

Three color STED and confocal image of a mammalian cultured cell immunostained for a nuclear pore protein in green and two golgi apparatus markers in red and blue.

abberior STAR RED highlights the golgi apparatus protein giantin (red) and abberior STAR ORANGE visualizes the cis-golgi protein GM130 (blue). NUP98 proteins were stained with abberior STAR GREEN (green).

Three color confocal image of human fibroblast immunostained with abberior STAR 580 for vimentin (green) and with abberior STAR RED for tubulin (red).

DAPI was added to highlight the nucleus (blue).

Four color confocal image of mammalian cultured cells. Peroxisomes were immunostained with abberior STAR RED (magenta) and nuclear pore proteins with abberior STAR 580 (green). F-actin fibers were highlighted with abberior STAR 488 phalloidin (gray).

The nucleus was visualized with DAPI (blue).