Perfect 3-color combination for STED @ 775 nm. abberior STAR RED NUP98 (red) and STAR ORANGE vimentin (cyan) complemented by the new STAR 460L tubulin (yellow). Cultured mammalian cells were fixed and labeled by indirect immunofluorescence.

We are happy to introduce the first commercial long Stokes-shift dye for live-cell imaging applications. The NEW abberior LIVE 460L makes 3-color live-cell STED at 775 nm possible!

Live-cell STED image of a cell expressing a SNAP-tag® in an outer mitochondrial membrane protein visualized by our abberior LIVE 460L SNAP (orange). Tubulin filaments are highlighted with LIVE 610 (cyan) and the DNA with LIVE 560 DNA (red).

STED time-lapse of living mammalian cell stained with abberior STAR ORANGE membrane.

Two color live-cell STED and confocal image of a mammalian cultured cell stained with abberior STAR RED membrane (orange) and abberior LIVE 590 actin (cyan).

Comparison of STED and confocal of a living mammalian cell stained with abberior STAR ORANGE membrane.

Comparison of STED and confocal of a living mammalian cell stained with abberior STAR 488 membrane. Images were acquired with our FACILITY and STED @ 595 nm.

Confocal time-lapse of living mammalian cell stained with abberior STAR 488 membrane.

abberior CAGE 552 is a caged dye which is initially nonfluorescent and colorless substance which, upon photolysis with UV light, readily transforms irreversibly into a red and highly fluorescent dye.

The cytoskeleton is visible after UV induced uncaging of abberior CAGE 552.

3D MINFLUX image of the mitochondrial import receptor Tom20 labeled with abberior FLUX 647 in fixed mammalian cells using indirect immunolabeling. Please note that Tom20 is only localized at the mitochondrial surface. The axial coordinate is color-coded.

Two-color MINFLUX revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cell labeled with indirect immunofluorescence using secondary antibodies coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.

MINFLUX imaging of βII spectrin in a primary hippocampal neuron labeled with abberior FLUX 680 by indirect immunofluorescence. Please note the periodic arrangement of spectrin along the axon.

MINFLUX image of axonal βII spectrin labeled with abberior FLUX 660 in primary hippocampal neurons. Note the periodic arrangement of spectrin along the axon, and the absence of any details in the confocal counterpart image.

2D MINFLUX nanoscopy of the nuclear pore complex subunits. Fixed mammalian cells expressing SNAP-tag® NUP96 were labeled with abberior FLUX 647 SNAP. In contrast to confocal microscopy, 2D MINFLUX allows to visualize the shape and arrangement of individual subunits of the nuclear pore complex.

2D MINFLUX imaging of the peroxisomal membrane protein PMP70 labeled with abberior FLUX 640 in fixed mammalian cells using indirect immunofluorescence.

Drosophila ovariole stained with abberior LIVE 560 DNA showing nuclei in different cell types of the egg chamber. Ovaries were dissected from adult female fruit flies and were fixed prior to staining.

Image was acquired with the STEDYCON tiling feature and assembled with the SVI Huygens Stitcher.

Drosophila spermatid tails were stained with abberior LIVE 610 tubulin. Testis were dissected from adult male fruit flies. Live cell imaging experiment was performed on a  INFINITY microscope.

Composite of three color live-cell image of an adherent mammalian cell. This living cell was directly labelled with abberior LIVE 510 actin (blue/green), LIVE 560 DNA (gray) and LIVE 610 tubulin. This image was acquired with a STEDYCON microscope.

Three color live-cell STED at 775 nm: living cell labelled with abberior LIVE 460L (ER, green), LIVE 560 tubulin (magenta) and LIVE 610 actin (red).