Confocal stitched image of a spheroid stained for nuclear pores (magenta), actin cytoskeleton (blue), and mitochondria (orange).

NIH-313 spheroid, fixed with 4% PFA, kindly provided by ibidi and prepared on µ-Slide VI 0.4 µ-Pattern ibiTreat.

Larva of Platynereis dumerilii acquired in a confocal overview. Nuclei are shown in cyan (DAPI), tubulin in magenta, and serotonin-positive neurons in yellow.

Confocal TIMEBOW acquisition of an Oleander leaf tip showing a range of autofluorescence lifetimes originating from different tissue components.

3 color confocal image of a Drosophila embryo stained for chitin (abberior STAR RED, cyan), tubulin (abberior STAR ORANGE, magenta), and DNA (PicoGreen, green).

Human cytomegalovirus particles in infected human foreskin fibroblasts, labeled for a viral glycoprotein (abberior STAR RED, blue) and a tegument protein (abberior STAR 580, green). The sample was 4x gel-expanded and imaged with STED.

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PFA-fixed mammalian cell stained by using Smart Secondaries^® from NanoTag Biotechnologies. Stained structures are intermediate filaments (cyan, abberior STAR 460L), mitochondria (green, abberior STAR GREEN), golgi (orange, abberior STAR ORANGE) and nuclear pore complex (magenta, abberior STAR RED).

MINFLUX uses information from the emission intensity of a fluorophore to constantly narrow the relative position of the excitation donut and the molecule. This adaptive correction process makes detected photons successively more informative. In contrast, other single-molecule localization techniques gather the exact same information from each detected photon. This way, MINFLUX boosts the summed spatial information gathered from a collection of photons and enables unprecedented resolution without pushing photon numbers or excitation exposure time.

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Dendrite with spines of a neuron grown on a coverslip, stained for βII spectrin and imaged in confocal mode and with the MINFLUX module of the MIRAVA POLYSCOPE.

Mitochondria labeled for the outer membrane protein TOMM20, imaged in confocal mode and with the MINFLUX module of the MIRAVA POLYSCOPE.

2D MINFLUX image of nuclear pore complex subunits, imaged in fixed mammalian cells expressing GFP-tagged NUP96 stained with abberior DNA-PAINT 660. The molecular resolution of the MINFLUX module of the MIRAVA POLYSCOPE allows visualizing the shape and arrangement of individual subunits of the nuclear pore complex.

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Confocal image of autofluorescence in a cross-section of the earthworm Lumbricus terrestris. TIMEBOW lifetime imaging detects differences in fluorescence lifetime, which depend on the type of autofluorescent molecule and its nano-environment, and visualizes them in distinct colors.

RAYSHAPE aberration correction allows you to look deep into complex samples without losing resolution or brightness due to aberrations. Deep tissue volume of Drosophila trachea showing chitin (autofluorescence) and actin (abberior STAR RED) deconvolved with TRUESHARP.

Confocal image of a developing zebrafish eye showing tubulin stained with abberior STAR RED and abberior STAR ORANGE.

Image courtesy of Graziamaria Paradisi, Marco Tartaglia, Antonella Lauri, Ospedale Pediatrico Bambino Gesù, Rome, Italy

Imaging across scales from diffraction-limited to molecular resolution: ribbon synapses in fixed mouse retina tissue stained for VAMP1 (abberior STAR RED) and CtBP2 (abberior STAR ORANGE) (confocal, MATRIX, STED) or for Bassoon (MINFLUX).

Sample courtesy: Arlene Hirano, PhD, University of California Los Angeles, Los Angeles, CA, USA (confocal, MATRIX, STED), and by Dr. Chad Grabner and Prof. Dr. Tobias Moser, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany (MINFLUX).

Video of mitochondria in living U2OS cells imaged for 1.5 hours with STED and deconvolved with TRUESHARP image boosting. Mitochondria were visualized with abberior LIVE SiR HaloX  staining the outer mitochondrial membrane marker TOMM20. FLEXPOSURE adaptive illumination was used to reduce the light burden on the sample.

A confocal image compared to a MATRIX + TRUESHARP STED image of nuclear pore complexes in mammalian cells stained for NUP96 with abberior STAR RED.

Living HeLa cells stained with the mitochondrial membrane marker abberior LIVE ORANGE mito, visualizing both outer and inner membranes. Confocal and STED images where deconvolved with TRUESHARP image boosting.

Confocal and STED image of a meiotic cell of Chinese spring wheat stained for two synaptonemal complex components.

Sample courtesy: Sepsi Adél, HUN-REN, Centre for Agricultural Research, Martonvásár, Hungary.

STED image of the golgi apparatus in a fixed mammalian cell, recorded with MATRIX array detection and deconvolved with TRUESHARP image boosting. Stained are the golgi proteins GM130 (cyan, abberior STAR RED) and giantin (orange, abberior STAR ORANGE).

Two-color 3D MINFLUX movie revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies) coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.

2D MINFLUX nanoscopy of the nuclear pore complex subunits, labeled with abberior FLUX 647 conjugated to JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies). In contrast to confocal microscopy, 2D MINFLUX allows visualization of the shape and arrangement of individual nuclear pore complex subunits. Here, we reach localization precisions of _~ 2 nm in raw localization data.

Two-color 3D MINFLUX revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies) coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.

Two color live-cell confocal and STED image of a mammalian cell directly labeled with abberior LIVE 510 mito (cyan), and LIVE RED tubulin. This image was acquired with a STEDYCON microscope.

A brain section stained for the neuronal proteins spectrin and adducine.

Overview image of the zebrafish olfactory epithelium.

Paraffin section of gut biopsy stained for Ki67 (abberior STAR ORANGE), Muc2 (abberior STAR RED), and DAPI.

Gain in both signal-to-background ratio and resolution: MATRIX detection dramatically improves a conventional STED image of the zebrafish olfactory epithelium, resulting in a perfect and crystal-clear image revealing even more detail than STED alone can.

xz section of a stage 17 Drosophila embryo stained for chitin (abberior LIVE 610, green) and DNA (abberior LIVE 550, cyan).

RAYSHAPE preserves resolution and brightness over the whole sample depth of about 200 µm by dynamically redirecting aberrated light to the right places.

In comparison, mechanical optics using a correction collar can only correct a limited z-range of approximately 20 µm

Drosophila stage 12 embryo, imaged with RAYSHAPE, stained for tubulin with abberior STAR RED and for DNA with abberior LIVE 550.

Deep tissue imaging with RAYSHAPE of a stage 17 Drosophila embryo stained for chitin with abberior LIVE 610.

Clear and detailed 3D rendering of trachea imaged with RAYSHAPE and MATRIX STED. Chitin was stained with abberior LIVE 610.

3D STED xz section of a Drosophila embryo trachea, imaged with RAYSHAPE and without abberation correction, depth 15 µm. Chitin was stained with abberior LIVE 610.

2D MINFLUX nanoscopy of Y. enterocolitica sorting platform protein Halo-YscL.

Sample from Alexander Carsten, Prof. Martin Aepfelbacher, Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Germany

STED-PAINT imaging of bacterial membranes (magenta) and DNA (green) using a high concentration of the exchangeable labels.

3D MINFLUX nanoscopy of Y. enterocolitica sorting platform protein Halo-YscL in xy view of two bacteria. The z-axis is color coded.

Sample from Alexander Carsten, Prof. Martin Aepfelbacher, Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Germany

Movement of fluorophore-labeled 30S ribosomes (colored) in two E. coli bacteria (grayscale) tracked with MINFLUX.

abberior STAR RED was coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch and used to label tubulin in fixed mammalian cells via indirect immunofluorescence. The STED image was acquired with the STEDYCON system.

Proteins of the nuclear pore complex and the golgi apparatus were stained by indirect immunofluorescence using abberior STAR RED (nuclear pore complex, magenta) and abberior STAR 580 (golgi, green) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.

Vimentin and tubulin were stained by indirect immunofluorescence using abberior STAR RED (vimentin, orange) and abberior STAR 580 (tubulin, cyan) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.

Indirect immunofluorescence staining with abberior STAR dyes coupled to polyclonal anti-mouse secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch shows excellent results in 3-color STED imaging. Staining was performed according to the premix & stain protocol. Vimentin (abberior STAR RED, green), tubulin (abberior STAR 580, magenta) and dsDNA (abberior STAR 460L, blue) were labeled in fixed mammalian cells and visualized with the STEDYCON.

OMP25-SNAP protein in mitochondria labeled with abberior LIVE 610 SNAP (magenta) and Actin^K118TAG labeled with abberior LIVE 550 click (green). Movie with 60 frames.

2-color STED image of a living cell expressing actin K118TAG incorporating TCO*A labeled with abberior LIVE 590 click (green). Plasma membrane is highlighted with abberior STAR RED membrane (magenta).

2-color STED image of a living cell expressing actin^K118TAG incorporating TCO*A labeled with abberior LIVE 550 click (cyan). Tubulin filaments were stained with our direct probe abberior LIVE 610 tubulin (yellow).