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Confocal image of autofluorescence in a cross-section of the earthworm Lumbricus terrestris. TIMEBOW lifetime imaging detects differences in fluorescence lifetime, which depend on the type of autofluorescent molecule and its nano-environment, and visualizes them in distinct colors.

RAYSHAPE aberration correction allows you to look deep into complex samples without losing resolution or brightness due to aberrations. Deep tissue volume of Drosophila trachea showing chitin (autofluorescence) and actin (abberior STAR RED) deconvolved with TRUESHARP.

Confocal image of a developing zebrafish eye showing tubulin stained with abberior STAR RED and abberior STAR ORANGE.

Image courtesy of Graziamaria Paradisi, Marco Tartaglia, Antonella Lauri, Ospedale Pediatrico Bambino Gesù, Rome, Italy

2D MINFLUX image of nuclear pore complex subunits, imaged in fixed mammalian cells expressing GFP-tagged NUP96 stained with abberior DNA-PAINT 660. The molecular resolution of MINFLUX allows visualizing the shape and arrangement of individual subunits of the nuclear pore complex.

U2OS cell line stably expressing NUP96 GFP provided by Prof. Dr. Jan Ellenberg and Dr. Jonas Ries, EMBL Heidelberg, Germany.

Imaging across scales from diffraction-limited to molecular resolution: ribbon synapses in fixed mouse retina tissue stained for VAMP1 (abberior STAR RED) and CtBP2 (abberior STAR ORANGE) (confocal, MATRIX, STED) or for Bassoon (MINFLUX).

Sample courtesy: Arlene Hirano, PhD, University of California Los Angeles, Los Angeles, CA, USA (confocal, MATRIX, STED), and by Dr. Chad Grabner and Prof. Dr. Tobias Moser, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany (MINFLUX).

Video of mitochondria in living U2OS cells imaged for 1.5 hours with STED and deconvolved with TRUESHARP image boosting. Mitochondria were visualized with abberior LIVE SiR HaloX  staining the outer mitochondrial membrane marker TOMM20. FLEXPOSURE adaptive illumination was used to reduce the light burden on the sample.

A confocal image compared to a MATRIX + TRUESHARP STED image of nuclear pore complexes in mammalian cells stained for NUP96 with abberior STAR RED.

Living HeLa cells stained with the mitochondrial membrane marker abberior LIVE ORANGE mito, visualizing both outer and inner membranes. Confocal and STED images where deconvolved with TRUESHARP image boosting.

Confocal and STED image of a meiotic cell of Chinese spring wheat stained for two synaptonemal complex components.

Sample courtesy: Sepsi Adél, HUN-REN, Centre for Agricultural Research, Martonvásár, Hungary.

Fixed cells stained for the golgi proteins GM130 (cyan, abberior STAR RED) and giantin (orange, abberior STAR ORANGE), recorded with MATRIX array detection, and deconvolved with TRUESHARP image boosting.

Two-color 3D MINFLUX movie revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies) coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.

2D MINFLUX nanoscopy of the nuclear pore complex subunits, labeled with abberior FLUX 647 conjugated to JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies). In contrast to confocal microscopy, 2D MINFLUX allows visualization of the shape and arrangement of individual nuclear pore complex subunits. Here, we reach localization precisions of _~ 2 nm in raw localization data.

Two-color 3D MINFLUX revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies) coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.

Two color live-cell confocal and STED image of a mammalian cell directly labeled with abberior LIVE 510 mito (cyan), and LIVE RED tubulin. This image was acquired with a STEDYCON microscope.

A brain section stained for the neuronal proteins spectrin and adducine.

Overview image of the zebrafish olfactory epithelium.