General Information

Storage

All abberior products are shipped at room temperature. For the period of shipment, even for several days, refrigeration of our products is not necessary.

Keep at 4 °C only for short-term storage. For long-term storage, we recommend a temperature of -20 °C to -80 °C.

We strongly recommend avoiding repeated freeze-thaw cycles.

Derivates/Phalloidins/Lipids

These products contain a vial of lyophilized powder and are shipped at room temperature. Upon arrival, please store the product at -20 °C. For proper long-term storage up to one year, protect the product from light and moisture.

Antibodies/Streptavidin/Neutravidin

These products contain a vial of buffered solution and are shipped at room temperature. Upon arrival, the product can be stored at 4 °C for up to one month. Please split the product into smaller aliquots and store these at -20 °C to -80 °C for long-term storage of up to one year. Protect the conjugate from direct light exposure and avoid repeated freeze-thaw cycles.

Short Description

Carboxylic acid

The dye without reactive groups can be activated at the carboxy group for coupling purposes. Our abberior carboxylic acid derivates come as a freeze-dried powder and should be dissolved in DMF, DMSO, or acetonitrile.

NHS ester/carbonate

The amino-reactive reagent N-hydroxysuccinimide (NHS) is a widespread tool to label primary amines of proteins (e.g. antibodies) or amine-modified molecules. This reactive group assists in forming a chemically stable bond between the dye and the protein. Our abberior NHS ester and carbonate are freeze-dried and should be dissolved only shortly before the coupling reaction. Recommended solvents include amine- and water-free DMF, DMSO, or acetonitrile. A detailed labeling protocol can be found here, on abberior. rocks.

Maleimide

For the labeling of thiol groups, the most widely used reagents are maleimides, which form a stable thioether bond with the protein. Our abberior maleimides are freeze-dried and should be dissolved only shortly before the coupling reaction. Recommended solvents include amine- and water-free DMF, DMSO, or acetonitrile. A detailed labeling protocol can be found here, on abberior. rocks.

Azide

The azide-alkyne Huisgen cycloaddition, also known as “Click Chemistry”, is commonly used to join small molecules together. Copper catalyzes the cycloaddition forming a covalent bond between label and molecule. Our abberior azide comes as a freeze-dried powder and should be dissolved in DMF or DMSO.

Phalloidin

Fluorescent dye conjugates of phalloidin probes are used for labeling, identifying, and quantifying F-actin filaments in formaldehyde-fixed and permeabilized cultured cells, whole organs, tissue sections, or plants. The high specificity of phalloidin probes offers significant advantages over antibodies for actin labeling. Our abberior phalloidin probes are freeze-dried and must be dissolved in DMF, DMSO, or methanol (abs.) upon arrival. For most applications, a staining solution of 1 Unit/ml is advised. However, the staining protocol may vary with cell type and application.  For more details on how to stain mammalian cultured cells with phalloidin see our white paper “Labeling for STED microscopy” or also here, on abberior.rocks.

Biotin

Fluorescent biotin derivatives are suitable for fluorescence labeling of avidin- or streptavidin-modified molecules. Our abberior biotin is a freeze-dried powder and should be dissolved in DMF or DMSO.

Lipids

Our abberior STAR membrane probes can be readily used to specifically label the outer plasma membrane of living cells or can be used for in vitro studies. All our bright and photostable abberior STAR membrane probes are modified by cholesterol as a functional group. Since the dye itself is not incorporated into the membrane, our unique membrane probes do not change the composition of the plasma membrane by foreign substances. The natural behavior of the membrane and fluidity will maintain.

Avidins

Streptavidin or neutravidin is suitable for fluorescence labeling of biotin-conjugated molecules. The product is dissolved in PBS pH 7,4 containing 2 mg/ml BSA to prevent denaturation of the conjugate. abberior streptavidin or neutravidin dye conjugates have a 1 mg/ml concentration. For most applications, a staining solution of 1 – 5 µg/ml can be used. However, staining protocols may vary with cell type and application.  

Antibodies

Our abberior secondary antibodies are excellent for indirect immunofluorescence techniques used for many light microscopy applications. The product is dissolved in PBS pH 7,4 containing 2 mg/ml BSA to prevent denaturation of the conjugate. abberior antibody conjugates have a concentration of 1 mg/ml. For most applications a dilution of 1:200 (5 µg/ml) is sufficient. However, the staining protocol may vary with cell type and application. For more details on how to stain mammalian cultured cells with abberior secondary antibodies see our white paper “Labeling for STED microscopy”.

Notice

• abberior products are reagents designed for in vitro scientific research use only and must not be used for diagnostic or therapeutic purposes or for use in humans.
• The use of abberior products must be supervised by technically qualified personnel experienced in handling potentially hazardous chemicals. For safety instructions please read the corresponding MSDS on www.abberior.shop
• Commercial use of abberior products is not permitted without written agreement by abberior GmbH.