Drosophila male accessory gland stained for F-actin using abberior STAR 580 phalloidin.

Sample was prepared in cooperation with Dr. H. R. Shcherbata at MPl for Biophysical Chemistry, Göttingen, Germany.

Three color STED and confocal image of a mammalian cultured cell immunostained for a nuclear pore protein in green and two golgi apparatus markers in red and blue.

abberior STAR RED highlights the golgi apparatus protein giantin (red) and abberior STAR ORANGE visualizes the cis-golgi protein GM130 (blue). NUP98 proteins were stained with abberior STAR GREEN (green).

Three color confocal image of human fibroblast immunostained with abberior STAR 580 for vimentin (green) and with abberior STAR RED for tubulin (red).

DAPI was added to highlight the nucleus (blue).

Four color confocal image of mammalian cultured cells. Peroxisomes were immunostained with abberior STAR RED (magenta) and nuclear pore proteins with abberior STAR 580 (green). F-actin fibers were highlighted with abberior STAR 488 phalloidin (gray).

The nucleus was visualized with DAPI (blue).

Drosophila female reproductive system stained for F-actin (red) with abberior STAR RED phalloidin. abberior STAR ORANGE is highlighting a nuclear pore protein (gray).

Image was acquired with the STEDYCON tiling feature and assembled with the SVI Huygens Stitcher.

Autofluorescence in a plant stem cross section of Convallaria recorded with FACILITY. A large area, 10 by 10 tiles, each 125 µm by 125 µm, was imaged and stitched with image tiling in Fiji for ImageJ. This is combined with TIMEBOW lifetime imaging to show the differences in fluorescence lifetime due to the type of autofluorescent molecule and their nano-environment.

Live-cell sample of an Arabidopsis root tip suspended in water recorded with FACILITY. A subset of cells expresses a YFP construct. Lifetime imaging with TIMEBOW shows shifts in YFP fluorescence lifetime caused by the proteins nano-environment.

We thank Dr. Fábián Attila and Soós Vilmos (ATK, Brunszvik, HU) for providing this sample. Contact: soos.vilmos@atk.hu.

Four-color staining of fixed mammalian cells recorded with FACILITY showing nuclear pores (NUP98, STAR RED), Golgi membrane (GM130, STAR 635), Golgi medial rims (Giantin, STAR ORANGE) and actin (STAR 580 phalloidin). Shown left, just using spectral information, STAR RED and STAR 635 (magenta) cannot be separated, because of their similar excitation properties. The same goes for STAR ORANGE and STAR 580 (grey).
With the lifetime information collected by TIMEBOW, these dyes can be distinguished, turning an image with two recording channels into a four-color image. To achieve superresolution only one STED laser at 775 nm was used.

All utilized dyes are manufactured by abberior.

Autofluorescence in a plant stem cross section of Convallaria recorded with FACILITY. Image quality is improved by removing the background through MATRIX detection. This is combined with TIMEBOW lifetime imaging to show the differences in fluorescence lifetime due to the type of autofluorescent molecule and their nano-environment.

TIMEBOW STED vs MATRIX + TIMEBOW STED

Actin in microvilli of CaCo2 cells recorded with FACILITY. Fixed cells were stained with abberior STAR RED phalloidin. Imaging of the intense-background sample is optimized by MATRIX detection by directly subtracting unfocussed data. To boost resolution TIMEBOW is used, keeping STED laser power low. Lifetime information enables the selective exclusion of STED-affected emission at the rim of each measurement.

We thank Prof. Dorothee Günzel and Jörg Piontek (Charité, Berlin, DE) for providing this sample.

Confocal vs MATRIX + TIMEBOW STED

Actin in microvilli of CaCo2 cells recorded with FACILITY. Fixed cells were stained with abberior STAR RED phalloidin. Imaging of the intense-background sample is optimized by MATRIX detection by directly subtracting unfocussed data. To boost resolution TIMEBOW is used, keeping STED laser power low. Lifetime information enables the selective exclusion of STED-affected emission at the rim of each measurement.

We thank Prof. Dorothee Günzel and Jörg Piontek (Charité, Berlin, DE) for providing this sample.

MINFLUX 3D imaging of βII spectrin in a primary hippocampal neuron labeled with Alexa Fluor 647 by indirect immunofluorescence. The axial coordinate is color-coded. Please note the periodic arrangement of spectrin along the axon.

MINFLUX 3D nanoscopy of nuclear pore complex subunits. 3D MINFLUX facilitates 3D imaging at molecular scales. In the axial direction localization, we reach precisions of approx. 2.5 nm in raw localization data. Labeling of NUP96-SNAP was performed using SNAP-Alexa Fluor 647.

3D MINFLUX imaging of the peroxisomal membrane protein PMP70 labelled with abberior FLUX 647 in fixed mammalian cells using indirect immunofluorescence. 3D MINFLUX allows visualizing the shape of peroxisomes in all directions.

MINFLUX 3D imaging of Clathrin coated vesicles and Clathrin coated pits. Labeling was performed using clathrin light chain-SNAP together with SNAP-Alexa Fluor 647.

MINFLUX image of the mitochondrial import receptor Tom20 labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunolabeling. Please note that Tom20 is only localized at the mitochondrial surface.

MINFLUX 3D on nuclear pore complexes. Localization precision is better than 3 nm along all directions

3D MINFLUX imaging of βII spectrin in a primary hippocampal neuron labeled with Alexa Fluor 647 by indirect immunofluorescence