3D MINFLUX allowes to visualize the full shape of peroxisomes.

2D MINFLUX nanoscopy of the nuclear pore complex subunits. NUP96-SNAP/SNAP-Alexa Fluor 647 lend themselves as benchmark structures to test superresolution light microscopes. In contrast to confocal microscopy, 2D MINFLUX allows to visualize the shape and arrangement of individual subunits of the nuclear pore complex. Here, we reach localization precisions of ~2 nm in raw localization data.

Two-color MINFLUX on mitochondria samples. The mitochondrial proteins TOM20 (green) and mtDNA (red) were labeled in mammalian cells with indirect immunofluorescence using secondary antibodies coupled to sCy5 and CF680. Two-color confocal (A) and MINFLUX (B) was performed using a ratiometric detection strategy. Please note that the labeling density of both structures is highly dissimilar. For TOM20 single proteins are labeled in the mitochondrial membrane, whereas numerous binding sites are decorated in the mtDNA. MINFLUX enables the visualization and separation of both structures.

Surface rendering of a confocal z-stack of a pollen grain.

Confocal and 2D STED imaging of dendrites in brain slices. GFP-tagged proteins were expressed and immunolabelled using primary antibodies against GFP and secondary antibodies coupled to abberior STAR 635P.

Sample was prepared by O. Kaplan and H. Kawabe @ MPI of Experimental Medicine, Göttingen, Germany.

Actin stain of mouse inner ear hair cells using abberior STAR RED phalloidin.

Samples were prepared by Dr. Christian Vogl, InnerEarLab, UMG Göttingen, Germany.

Movie through a confocal TIMEBOW 3D image stack of a pollen grain (z-coordinate is animated).

SARS-CoV2 ("Corona", magenta, abberior STAR RED) and cellular actin (green, abberior STAR ORANGE Phalloidin) in VeroE6 infected cells.

Copyright: MDVA team/CNRS Montpellier, France (D. Muriaux, C. Favard, M.-P. Blanchard @MRI)

Vero cell, vimentin is labeled with abberior STAR RED. Note how in areas, where vimentin is thick, MATRIX removes background, while keeping the fibers in the foreground intact.

Actin stain of mouse inner ear hair cells using abberior STAR RED phalloidin. Zoom in for full effect.

Samples were prepared by Dr. Christian Vogl, InnerEarLab, UMG Göttingen, Germany.

Nuclear pores with EASY3D STED. Background removal with MATRIX is particularly advantageous with 3D-STED.

Four xy-planes from a volume stack recording of nuclear pore complexes (NUPs). With MATRIX, nuclear pores that are not in focus are effectively removed from the image.

Cultured mammalian cells (NUP, Giantin, and Vimentin, labeled with abberior STAR RED, STAR ORANGE, STAR GREEN). Out-of-focus contributions of all three types of structures is effectively removed and section is improved.

Two proteins in the Golgi apparatus were immunolabelled using primary antibodies specific for GM130 and Giantin and secondary antibodies coupled to abberior STAR 580 and abberior STAR 635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.