Sample gallery
Fluorescence imaging, whether at confocal, STED or MINFLUX resolution, guarantees unique insights into the function and structure of life at the molecular level. Besides the scientific information content, some sample portraits provide simply beautiful images. Enjoy browsing our sample gallery.
the fine art of science
Description
Movie through a confocal TIMEBOW 3D image stack of a pollen grain (z-coordinate is animated).
Modules:
Description
SARS-CoV2 ("Corona", magenta, abberior STAR RED) and cellular actin (green, abberior STAR ORANGE Phalloidin) in VeroE6 infected cells.
Copyright: MDVA team/CNRS Montpellier, France (D. Muriaux, C. Favard, M.-P. Blanchard @MRI)
Description
Vero cell, vimentin is labeled with abberior STAR RED. Note how in areas, where vimentin is thick, MATRIX removes background, while keeping the fibers in the foreground intact.
Modules:
Description
Actin stain of mouse inner ear hair cells using abberior STAR RED phalloidin. Zoom in for full effect.
Samples were prepared by Dr. Christian Vogl, InnerEarLab, UMG Göttingen, Germany.
Modules:
Description
Four xy-planes from a volume stack recording of nuclear pore complexes (NUPs). With MATRIX, nuclear pores that are not in focus are effectively removed from the image.
Modules:
Description
Cultured mammalian cells (NUP, Giantin, and Vimentin, labeled with abberior STAR RED, STAR ORANGE, STAR GREEN). Out-of-focus contributions of all three types of structures is effectively removed and section is improved.
Modules:
Description
Two proteins in the Golgi apparatus were immunolabelled using primary antibodies specific for GM130 and Giantin and secondary antibodies coupled to abberior STAR 580 and abberior STAR 635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.
Description
Vero cells, Vimentin (green, abberior STAR Red) and Phalloidin (white, abberior STAR Orange)
Modules:
Description
Centrosome linker. U2OS cells in which the centrosome-linker-protein rootletin was immunolabelled using secondary antibodies coupled to abberior STAR RED. The sample was prepared by R. Vlijm at MPI for Medical Research, Heidelberg, Germany. Imaged with abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Description
Primary hippocampal neurons with cytoskeleton proteins labeled (magenta, alpha-Adducin, abberior STAR 635P and green, ßII spectrin, Alexa 594). Imaged with abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Maximum intensity projection of a z-stack showing nuclear pore complex protein (red, abberior STAR RED) and peroxisomes (cyan, Alexa 594) in mammalian cells. Imaged with abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Actin in microvilli of Caco2 cells. Fixed cells were stained with abberior STAR RED phalloidin. MATRIX detection removes background signal and dramatically improves optical sectioning for 2D-STED.
Samples by Prof. Dorothee Günzel & Roman Mannweiler, Institut für Klinische Physiologie, Charité - Universitätsmedizin Berlin.
Modules:
Description
Body wall preparation of a 3rd instar Drosophila melanogaster larva. Synapses at the neuromuscular junction are visualized by immunostaining against Bruchpilot (Brp), an integral component of active zones in Drosophila. Images were acquired on a STEDYCON attached to an Olympus IX81. Images were kindly provided by Dr. Nadine Ehmann, Institute of Physiology, Neurophysiology, University of Würzburg.
Description
Pollen autofluorescence imaged with a STEDYCON on an IX83 microscope frame. z-stacks on 95 tiles were acquired and stitched with SVI Huygens.
Description
Immunolabelling of βIV-spectrin, a scaffolding protein of the axon initial segment, using a self-made primary antibody and Alexa 594 secondary antibody. Cryo-section of mouse neocortex cut at 20 µm. Images were acquired using a STEDYCON attached to a Zeiss AxioImager Z2. The sample was kindly provided by Dr. Maren Engelhardt, Institute of Neuroanatomy, Medical Faculty Mannheim, Heidelberg University.
Description
Confocal imaging of a large sample region. Shown is a 2.8 mm x 2.5 mm region, acquired using 35 by 29 tiles using the STEADYFOCUS and stitched with SVI Huygens. Sample: mammalian cells immunolabelled for the mitochondrial protein TOM20 (abberior STAR RED, red), double-stranded DNA to visualize mitochondrial DNA (abberior STAR ORANGE, green), phalloidin to label F-actin (abberior STAR GREEN, gray), and DAPI to label nuclei. Sample preparation by abberior GmbH.
Description
Movie through a confocal TIMEBOW 3D image stack of a pollen grain (z-coordinate is animated).
Modules:
Description
SARS-CoV2 ("Corona", magenta, abberior STAR RED) and cellular actin (green, abberior STAR ORANGE Phalloidin) in VeroE6 infected cells.
Copyright: MDVA team/CNRS Montpellier, France (D. Muriaux, C. Favard, M.-P. Blanchard @MRI)
Description
Vero cell, vimentin is labeled with abberior STAR RED. Note how in areas, where vimentin is thick, MATRIX removes background, while keeping the fibers in the foreground intact.
Modules:
Description
Actin stain of mouse inner ear hair cells using abberior STAR RED phalloidin. Zoom in for full effect.
Samples were prepared by Dr. Christian Vogl, InnerEarLab, UMG Göttingen, Germany.
Modules:
Description
Nuclear pores with EASY3D STED. Background removal with MATRIX is particularly advantageous with 3D-STED.
Modules:
Description
Four xy-planes from a volume stack recording of nuclear pore complexes (NUPs). With MATRIX, nuclear pores that are not in focus are effectively removed from the image.
Modules:
Description
Cultured mammalian cells (NUP, Giantin, and Vimentin, labeled with abberior STAR RED, STAR ORANGE, STAR GREEN). Out-of-focus contributions of all three types of structures is effectively removed and section is improved.
Modules:
Description
Two proteins in the Golgi apparatus were immunolabelled using primary antibodies specific for GM130 and Giantin and secondary antibodies coupled to abberior STAR 580 and abberior STAR 635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.
Description
Vero cells, Vimentin (green, abberior STAR Red) and Phalloidin (white, abberior STAR Orange)
Modules:
Description
Centrosome linker. U2OS cells in which the centrosome-linker-protein rootletin was immunolabelled using secondary antibodies coupled to abberior STAR RED. The sample was prepared by R. Vlijm at MPI for Medical Research, Heidelberg, Germany. Imaged with abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Description
Primary hippocampal neurons with cytoskeleton proteins labeled (magenta, alpha-Adducin, abberior STAR 635P and green, ßII spectrin, Alexa 594). Imaged with abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Maximum intensity projection of a z-stack showing nuclear pore complex protein (red, abberior STAR RED) and peroxisomes (cyan, Alexa 594) in mammalian cells. Imaged with abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Actin in microvilli of Caco2 cells. Fixed cells were stained with abberior STAR RED phalloidin. MATRIX detection removes background signal and dramatically improves optical sectioning for 2D-STED.
Samples by Prof. Dorothee Günzel & Roman Mannweiler, Institut für Klinische Physiologie, Charité - Universitätsmedizin Berlin.
Modules:
Description
Body wall preparation of a 3rd instar Drosophila melanogaster larva. Synapses at the neuromuscular junction are visualized by immunostaining against Bruchpilot (Brp), an integral component of active zones in Drosophila. Images were acquired on a STEDYCON attached to an Olympus IX81. Images were kindly provided by Dr. Nadine Ehmann, Institute of Physiology, Neurophysiology, University of Würzburg.
Description
Pollen autofluorescence imaged with a STEDYCON on an IX83 microscope frame. z-stacks on 95 tiles were acquired and stitched with SVI Huygens.
Description
Immunolabelling of βIV-spectrin, a scaffolding protein of the axon initial segment, using a self-made primary antibody and Alexa 594 secondary antibody. Cryo-section of mouse neocortex cut at 20 µm. Images were acquired using a STEDYCON attached to a Zeiss AxioImager Z2. The sample was kindly provided by Dr. Maren Engelhardt, Institute of Neuroanatomy, Medical Faculty Mannheim, Heidelberg University.
Description
Confocal imaging of a large sample region. Shown is a 2.8 mm x 2.5 mm region, acquired using 35 by 29 tiles using the STEADYFOCUS and stitched with SVI Huygens. Sample: mammalian cells immunolabelled for the mitochondrial protein TOM20 (abberior STAR RED, red), double-stranded DNA to visualize mitochondrial DNA (abberior STAR ORANGE, green), phalloidin to label F-actin (abberior STAR GREEN, gray), and DAPI to label nuclei. Sample preparation by abberior GmbH.