TIMEBOW confocal and TIMEBOW STED image of mammalian cells labeled with antibodies against lamin, ATTO 647N and dsDNA, abberior STAR 635P.

2D tracking of a lipid-coupled Atto 647N molecule embedded in a supported lipid bilayer. The movement of the labeled lipid was tracked with a frequency of up to 10 kHz.

2D MINFLUX imaging of the cytoskeletal protein vimentin. Vimentin was labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunofluorescence. Note the individual filaments at intersections are invisible in the confocal image.

2D MINFLUX imaging of the peroxisomal membrane protein PMP70 labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunofluorescence

Two-color confocal and MINFLUX images of Tom20 (green) and mitochondrial DNA (red) stained with sCy5 and CF680 in mammalian cells using indirect immunolabeling. The two fluorophores were distinguished by ratiometric detection strategy. Note the dissimilar labeling density of the two imaged structures.

2D MINFLUX image of the mitochondrial import receptor Tom20 labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunolabeling.

MINFLUX image of axonal bII spectrin in primary hippocampal neurons with < 2 nm resolution. Note the periodic arrangement of spectrin along the axon, and the absence of any details in the confocal counterpart image.

Tom20 (red) and golgi (blue) in cultured mammalian cells.

Nuclear pore complex (green), Tom20 (red), and vimentin (white) in cultured mammalian cells.

Actin stain of mouse inner ear hair cells using abberior STAR RED phalloidin.

Samples were prepared by Dr. Christian Vogl, InnerEarLab, UMG Göttingen, Germany.

2-color 2D STED image of a cleared adult kidney sample of a rat. Shown is an image of a renal corpuscle showing Nephrin (red, abberior STAR 635P) structures inbetween the Podocin slits (green, AlexaFluor594).

Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.

4-color confocal image of mammalian cells (DAPI, Phalloidin, Tubulin, Tom20).

Growth cone at the tip of the axon of a primary hippocampal neuron at 1 day in vitro. Microtubules (Tuj1, abberior STAR 580, red) are bundled in the central-domain suggesting a pausing state. The molecular motor myosin IIB (confocal, Alexa488, blue) is enriched at the transition-zone, along the F-actin arcs. In the peripheral domain actin forms bundles in the filopodia (Phalloidin, abberior STAR 635, green).

3-color STED image of primary hippocampal neurons. Please note the characteristic ~190 nm beta II spectrin periodicity along distal axons (green) which is only visible in the STED image. Labelled structures: beta II spectrin (green, abberior STAR 635P), Bassoon (red, abberior STAR 580), Actin cytoskeleton (blue, phalloidin, Oregon Green 488). Imaged with abberior Expert Line with 595nm and 775nm STED laser. Sample was prepared by Elisa D’Este @ MPIBPC, Göttingen.