Sample gallery
Fluorescence imaging, whether at confocal, STED or MINFLUX resolution, guarantees unique insights into the function and structure of life at the molecular level. Besides the scientific information content, some sample portraits provide simply beautiful images. Enjoy browsing our sample gallery.
the fine art of science
Description
TIMEBOW image of tubulin labeled with Atto647N and vimentin labeled with abberior STAR 635P. The two labels (Atto647N and abberior STAR 635P) have the same excitation spectra, but different lifetimes. Tubulin (red, short lifetime) and vimentin (green, long lifetime) can successfully be separated in the TIMEBOW STED recording via their lifetimes.
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Description
TIMEBOW image of mammalian cells labeled with antibodies against Tom20, ATTO 647N and NUP153, abberior STAR 635P. Note that nuclear pore (NUP) complex subunits appear to be localized in the cytoplasm, which is due to the NUP import pathway into the nuclear membrane.
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Description
TIMEBOW confocal and TIMEBOW STED image of mammalian cells labeled with antibodies against lamin, ATTO 647N and dsDNA, abberior STAR 635P.
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Description
2D tracking of a lipid-coupled Atto 647N molecule embedded in a supported lipid bilayer. The movement of the labeled lipid was tracked with a frequency of up to 10 kHz.
Description
2D MINFLUX imaging of the cytoskeletal protein vimentin. Vimentin was labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunofluorescence. Note the individual filaments at intersections are invisible in the confocal image.
Description
2D MINFLUX imaging of the peroxisomal membrane protein PMP70 labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunofluorescence
Description
Two-color confocal and MINFLUX images of Tom20 (green) and mitochondrial DNA (red) stained with sCy5 and CF680 in mammalian cells using indirect immunolabeling. The two fluorophores were distinguished by ratiometric detection strategy. Note the dissimilar labeling density of the two imaged structures.
Description
2D MINFLUX image of the mitochondrial import receptor Tom20 labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunolabeling.
Description
MINFLUX image of axonal bII spectrin in primary hippocampal neurons with < 2 nm resolution. Note the periodic arrangement of spectrin along the axon, and the absence of any details in the confocal counterpart image.
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Nuclear pore complex (green), Tom20 (red), and vimentin (white) in cultured mammalian cells.
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Description
Actin stain of mouse inner ear hair cells using abberior STAR RED phalloidin.
Samples were prepared by Dr. Christian Vogl, InnerEarLab, UMG Göttingen, Germany.
Description
2-color 2D STED image of a cleared adult kidney sample of a rat. Shown is an image of a renal corpuscle showing Nephrin (red, abberior STAR 635P) structures inbetween the Podocin slits (green, AlexaFluor594).
Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.
Description
TIMEBOW image of tubulin labeled with Atto647N and vimentin labeled with abberior STAR 635P. The two labels (Atto647N and abberior STAR 635P) have the same excitation spectra, but different lifetimes. Tubulin (red, short lifetime) and vimentin (green, long lifetime) can successfully be separated in the TIMEBOW STED recording via their lifetimes.
Modules:
Description
TIMEBOW image of mammalian cells labeled with antibodies against Tom20, ATTO 647N and NUP153, abberior STAR 635P. Note that nuclear pore (NUP) complex subunits appear to be localized in the cytoplasm, which is due to the NUP import pathway into the nuclear membrane.
Modules:
Description
TIMEBOW confocal and TIMEBOW STED image of mammalian cells labeled with antibodies against lamin, ATTO 647N and dsDNA, abberior STAR 635P.
Modules:
Description
2D tracking of a lipid-coupled Atto 647N molecule embedded in a supported lipid bilayer. The movement of the labeled lipid was tracked with a frequency of up to 10 kHz.
Description
2D MINFLUX imaging of the cytoskeletal protein vimentin. Vimentin was labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunofluorescence. Note the individual filaments at intersections are invisible in the confocal image.
Description
2D MINFLUX imaging of the peroxisomal membrane protein PMP70 labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunofluorescence
Description
Two-color confocal and MINFLUX images of Tom20 (green) and mitochondrial DNA (red) stained with sCy5 and CF680 in mammalian cells using indirect immunolabeling. The two fluorophores were distinguished by ratiometric detection strategy. Note the dissimilar labeling density of the two imaged structures.
Description
2D MINFLUX image of the mitochondrial import receptor Tom20 labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunolabeling.
Description
MINFLUX image of axonal bII spectrin in primary hippocampal neurons with < 2 nm resolution. Note the periodic arrangement of spectrin along the axon, and the absence of any details in the confocal counterpart image.
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Nuclear pore complex (green), Tom20 (red), and vimentin (white) in cultured mammalian cells.
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Description
Actin stain of mouse inner ear hair cells using abberior STAR RED phalloidin.
Samples were prepared by Dr. Christian Vogl, InnerEarLab, UMG Göttingen, Germany.
Modules:
Description
2-color 2D STED image of a cleared adult kidney sample of a rat. Shown is an image of a renal corpuscle showing Nephrin (red, abberior STAR 635P) structures inbetween the Podocin slits (green, AlexaFluor594).
Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.