MIRAVA POLYSCOPE

FAQ Video 43

“MIRAVA POLYSCOPE – one for all and all for one: the perfect picture”

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FAQ Video 44

“MIRAVA POLYSCOPE – watch the premiere of the world’s only all-in-one solution for confocal, MATRIX, STED, and MINFLUX microscopy”

Watch >

All-in-one solution

for confocal, MATRIX, STED, and MINFLUX microscopy

With MIRAVA, enjoy the ultimate combination of performance and ease-of-use. Carry out ambitious yet straightforward confocal imaging with down to 200 nm resolution. Take a step beyond the diffraction limit with the MATRIX array detector and record more detailed, background-free views of your sample. Activate STED (stimulated emission depletion) with a few clicks for high-end superresolution in three dimensions. And perform single molecule localization microscopy using MINFLUX (minimal fluorescence photon fluxes), with a localization precision down to 3.2 nm.

Each of abberior’s modules adds another superpower to your MIRAVA POLYSCOPE to give you exactly what you need to acquire the perfect image.

Adapt your system with the most advanced solutions to meet your research demands – whether it is sharper imaging deep in the specimen, physical background removal, versatile lifetime imaging, reduced light burden, or next-level deconvolution for the finishing touches.

All these technologies can be easily applied with our elegant and intuitive LiGHTBOX software. It guides new users to their first stunning images within minutes while experts enjoy a wide range of functions.

Ingenious engineering meets fancy physics.

The MIRAVA POLYSCOPE unites all our expertise and experience in a single instrument. It sets new standards in terms of optical quality and robustness. Due to the single-digit nanometer precision requirements for MINFLUX imaging, our MIRAVA POLYSCOPE is ten times more stable than traditional confocal and STED microscopes.

Every single component, from the first laser to the last lens, has been refined to run in perfect harmony. And the modularized hardware simplifies the installation of new components in the field, making it a future-proof choice.

^{Sample courtesy: Arlene Hirano, PhD, University of California Los Angeles, Los Angeles, CA, USA (confocal, MATRIX, STED), and Dr. Chad Grabner and Prof. Dr. Tobias Moser, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany (MINFLUX).}

Overview
up to 125 x 125 µm

Confocal
down to 200 nm

MATRIX
down to 70 nm

STED
down to 20 nm

MINFLUX
down to 3.2 nm

Focus on your research, we take care of the rest

Confocal imaging

Resolution down to 200 nm

MIRAVA’s superpowers

MINFLUX nanoscale imaging

MATRIX array detection

TRUESHARP image boosting

TIMEBOW lifetime imaging

FLEXPOSURE adaptive illumination

RAYSHAPE aberration correction

LiGHTBOX software

The MIRAVA POLYSCOPE takes confocal imaging to the next level.

With an extensive range of state-of-the-art features, you will enjoy unparalleled performance that meets the highest of imaging demands. No matter what your project is, be it live cell, deep tissue, or multicolor imaging, MIRAVA empowers users to achieve their goals with unmatched comfort and flexibility.

Equipped with seven excitation laser lines, MIRAVA guarantees straightforward and versatile multicolor imaging. It’s exceptionally gentle to the sample: our avalanche photodiode (APD) detectors require only half the excitation power to collect the same signal as hybrid detectors.²⁾ With abberior’s unique module for adaptive illumination we take it to the next level of sample care. With FLEXPOSURE you will bring down the light dose by up to orders of magnitude, without compromising on contrast and resolution.

Apply one or more of MIRAVA’s many modules to create the perfect image for your needs: get rid of the haze while enhancing resolution with our MATRIX array detector. Dive deeper into complex samples without worrying about aberrations eating up your signal, thanks to RAYSHAPE dynamic aberration correction. Use TIMEBOW to add the dimension of fluorescence lifetime to your experiment, giving you more information about your sample on many levels.

And if you require more detail, you can simply switch to STED or MINFLUX technology without needing to switch to another microscope. That’s imaging across all scales with MIRAVA.

^{Sample courtesy: Arlene Hirano, PhD, University of California Los Angeles, Los Angeles, CA, USA (confocal, MATRIX, STED), and Dr. Chad Grabner and Prof. Dr. Tobias Moser, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany (MINFLUX).}

Overview
up to 125 x 125 µm

Confocal
down to 200 nm

MATRIX
down to 70 nm

STED
down to 20 nm

MINFLUX
down to 3.2 nm

Many eyes see more than one

MATRIX imaging

Resolution down to 70 nm

MIRAVA’s superpowers

MINFLUX nanoscale imaging

MATRIX array detection

TRUESHARP image boosting

TIMEBOW lifetime imaging

FLEXPOSURE adaptive illumination

RAYSHAPE aberration correction

LiGHTBOX software

Imaging with MATRIX array detection means resolution beyond the diffraction limit and unsurpassed signal-to-background ratio.

Thick or dense samples with a lot of background become clear and sharp as the MATRIX isolates relevant signal from the rest. This allows you to process and analyze data with much greater confidence and achieve the best results.

Our MATRIX detector consists of 23 individual detector elements. On its own, each one of them is a small pinhole, able to record at the highest diffraction-limited resolution. At the same time, all elements taken together, give you the full signal of a large-area detector. The best out of both worlds, high signal and high resolution in one single shot!

And it gets even better. A single detector, such as a photomultiplier or a hybrid detector, collects signal from the focal plane, but also from other planes above and below. It cannot tell whether a detected photon is wanted in-focus light or irritating background.

In contrast, each of the many MATRIX detector elements looks at the sample from a different angle, allowing for precise differentiation between in-focus and out-of-focus signals. The result is crystal-clear images with an unparalleled signal-to-background ratio, even in challenging imaging conditions.

MATRIX becomes even more powerful when paired with TRUESHARP image boosting, abberior’s answer to the challenge of reliable deconvolution.

In contrast to common deconvolution algorithms, TRUESHARP works without a-priori assumptions to remove noise and background and to enhance resolution. Instead, it uses measured information from the image itself, such as data recorded with MATRIX or TIMEBOW. That way, TRUESHARP gets rid of disturbances and pushes resolution without falsifying the image.

Enjoy 100% measured superresolution and stunning signal-to-background ratio with MATRIX.

^{Sample courtesy: Arlene Hirano, PhD, University of California Los Angeles, Los Angeles, CA, USA (confocal, MATRIX, STED), and Dr. Chad Grabner and Prof. Dr. Tobias Moser, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany (MINFLUX).}

Overview
up to 125 x 125 µm

Confocal
down to 200 nm

MATRIX
down to 70 nm

STED
down to 20 nm

MINFLUX
down to 3.2 nm

Nobel prize technology from its inventors

STED imaging

Resolution down to 20 nm

MIRAVA’s superpowers

MINFLUX nanoscale imaging

MATRIX array detection

TRUESHARP image boosting

TIMEBOW lifetime imaging

FLEXPOSURE adaptive illumination

RAYSHAPE aberration correction

LiGHTBOX software

MIRAVA STED – 30 years of experience meets attention to detail.

Performing STED microscopy on the MIRAVA means applying top-notch technology for best results. For low to medium resolutions, use gentle STED laser power together with TRUESHARP image boosting for a significant increase in resolution compared to confocal imaging, without stressing the sample. Increase power if you need an even more detailed image and use FLEXPOSURE adaptive illumination to keep the light dose down. Or push it to the max and combine all our STED technologies to reach 20 nm resolution. Whatever you need, with MIRAVA, it’s all just a matter of a few clicks.

Even 3D STED is no more difficult. abberior’s dynamic beam shaper modulates the STED donut and optimally adapts it to different objective lenses.

abberior’s superpowers to master any task.

RAYSHAPE dynamic aberration correction allows you to look deep into complex samples like tissue or whole organisms without compromising on image quality due to aberrations. Using adaptive optics with a deformable mirror, RAYSHAPE puts diverted rays back on track, guaranteeing bright and crisp images from top to bottom – regardless of embedding medium, immersion medium, or specimen.

We established pulsed STED lasers as a gold standard long ago to reduce bleaching and phototoxicity and further reduced these harmful effects with the development of FLEXPOSURE adaptive illumination. With FLEXPOSURE, you are still recording long-term experiments over volumes or many frames, while ordinary always-on-lasers have long bleached the sample to death. Instead of illuminating the specimen with full intensity everywhere, we are gentle to the sample and turn all lasers on and off, shining light only where and when it has an effect and nowhere else.

TIMEBOW lifetime imaging gives time a color. Every fluorescence signal contains lifetime information that travels with the photons from the sample to the detector. TIMEBOW allows you to reveal previously hidden structural details by separating fluorophores emitting in the same spectral channel. This gives you great additional possibilities when it comes to multicolor labeling and imaging. Additionally, TIMEBOW STED exploits the spatial information encoded in the lifetime to increase image resolution.

Add the MATRIX array detector and increase resolution and image quality even further, by using the information that is encoded in the simultaneous recording of 23 images from different angles.

Our wonder weapon is TRUESHARP image boosting. TRUESHARP offers next generation deconvolution you can trust. It removes noise and background signal from your recorded data and strongly enhances resolution, sharpness, and brilliance of your images by incorporating experimentally measured information.

Of course, you can combine the capabilities of all modules for getting the perfect picture.

^{Sample courtesy: Arlene Hirano, PhD, University of California Los Angeles, Los Angeles, CA, USA (confocal, MATRIX, STED), and Dr. Chad Grabner and Prof. Dr. Tobias Moser, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany (MINFLUX).}

Overview
up to 125 x 125 µm

Confocal
down to 200 nm

MATRIX
down to 70 nm

STED
down to 20 nm

MINFLUX
down to 3.2 nm

100x sharper than a confocal

MINFLUX imaging

Resolution down to 3.2 nm

MIRAVA’s superpowers

MINFLUX nanoscale imaging

MATRIX array detection

TRUESHARP image boosting

TIMEBOW lifetime imaging

FLEXPOSURE adaptive illumination

RAYSHAPE aberration correction

LiGHTBOX software

Highest resolution at the lowest light dose, accessible to every researcher.

The MINFLUX module of the MIRAVA POLYSCOPE delivers nanometer-scale resolution that was previously achievable only with electron microscopes, but with the gentler touch of light.

MINFLUX stands out in the single molecule localization microscopy field. Other techniques follow the principle “the more, the merrier” and require huge numbers of fluorescence photons to achieve maximum resolution. MINFLUX, in contrast, localizes fluorophores by finding out where they do
not emit when excited with a hollow beam – minima-seeking, not maxima-bleaching. Our MIRAVA POLYSCOPE achieves molecular resolutions of a few nanometers without pushing the photon budget.

From sample preparation to imaging and analysis, MINFLUX uses simple, well established workflows. Labeling works as with any other type of single molecule localization microscopy.

Since abberior always takes a holistic approach, we offer FLUX dyes that are designed for superior performance in MINFLUX imaging applications.

They combine exceptional photostability, very high brightness, and low background labeling with photoswitching kinetics optimized for MINFLUX imaging. Conjugates of our dyes show excellent solubility in aqueous buffers and allow background-free labeling in cells or tissue.

Straightforward, easy, and reliable: unmatched resolution out-of-the-box with MINFLUX.

With the MINFLUX module of the MIRAVA POLYSCOPE, imaging at the molecular level is easier than ever. Set up and control your MINFLUX measurement with just a few clicks, thanks to seamless integration into our sleek LiGHTBOX software.

The MINFLUX hardware is fully integrated into the MIRAVA system, yielding unmatched robustness and optical quality. It constantly monitors and corrects even subtle, sub-nanometer drifts caused by environmental changes (e.g. ambient temperature), keeping your sample perfectly still for days.

MIRAVA sets the stage for effortless microscopy on the molecular level. Focus on your measurement and let MIRAVA do the MINFLUX.

^{3D STED volume of microglia and processes labeled for a specific cell surface receptor in mouse neocortical tissue. Sample courtesy: Csaba Cserép and Ádám Dénes, Laboratory of Neuroimmunology, HUN-REN Institute of Experimental Medicine, Budapest, Hungary}