2D MINFLUX image of the mitochondrial import receptor Tom20 labeled with Alexa Fluor 647 in fixed mammalian cells using indirect immunolabeling.

MINFLUX image of axonal bII spectrin in primary hippocampal neurons with < 2 nm resolution. Note the periodic arrangement of spectrin along the axon, and the absence of any details in the confocal counterpart image.

Tom20 (red) and golgi (blue) in cultured mammalian cells.

Nuclear pore complex (green), Tom20 (red), and vimentin (white) in cultured mammalian cells.

Actin stain of mouse inner ear hair cells using abberior STAR RED phalloidin.

Samples were prepared by Dr. Christian Vogl, InnerEarLab, UMG Göttingen, Germany.

2-color 2D STED image of a cleared adult kidney sample of a rat. Shown is an image of a renal corpuscle showing Nephrin (red, abberior STAR 635P) structures inbetween the Podocin slits (green, AlexaFluor594).

Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.

4-color confocal image of mammalian cells (DAPI, Phalloidin, Tubulin, Tom20).

Growth cone at the tip of the axon of a primary hippocampal neuron at 1 day in vitro. Microtubules (Tuj1, abberior STAR 580, red) are bundled in the central-domain suggesting a pausing state. The molecular motor myosin IIB (confocal, Alexa488, blue) is enriched at the transition-zone, along the F-actin arcs. In the peripheral domain actin forms bundles in the filopodia (Phalloidin, abberior STAR 635, green). Sample courtesy: Elisa D'Este, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.

3-color STED image of primary hippocampal neurons. Please note the characteristic ~190 nm beta II spectrin periodicity along distal axons (green) which is only visible in the STED image. Labelled structures: beta II spectrin (green, abberior STAR 635P), Bassoon (red, abberior STAR 580), Actin cytoskeleton (blue, phalloidin, Oregon Green 488). Imaged with abberior Expert Line with 595nm and 775nm STED laser. Sample was prepared by Elisa D’Este @ MPIBPC, Göttingen.

3-color STED imaging: active zones at the Drosophila larval neuromuscular junction immunostained for Bruchpilot and two other proteins.

Two superresolution channels (magenta, yellow) using a 775nm STED laser & one superresolution channel using a 595nm STED laser.

Samples by M. Lenz & M. Landgraf (University of Cambridge, UK).

Spherical aberrations arising from index mismatch between sample and immersion are corrected for, as well as higher-order, sample-induced aberrations.
Without Adaptive Optics, excitation laser power is typically increased with focus depth in order to compensate loss of signal due to aberrations. Adaptive Optics preserves resolution and brightness deep inside thick samples and enables imaging at low light levels.

Sample courtesy of Amelie Cabirol and Albrecht Haase, University of Trento.

Confocal acquisition of Convallaria rhizome (cross-section) with a 20x oil objective; 3 channels, maximum intensity projection. The image consists of 14 z-planes in 9 by 9 tile pattern comprising a total area of 3.2 mm x 3.2 mm, stitched using SVI Huygens. Shown is a maximum intensity projection.

Drosophila ovariole stained with abberior LIVE 560 DNA showing nuclei in different cell types of the egg chamber. Ovaries were dissected from adult female fruit flies and were fixed prior to staining.

Image was acquired with the STEDYCON tiling feature and assembled with the SVI Huygens Stitcher.