Adaptive optics EASY3D enables deep imaging of cleared adult kidney samples. Comparison of a XZ-slice deep into a renal corpuscle using conventional 3D STED imaging and an EASY3D imaging. EASY3D allowed imaging up to 80 µm into cleared rat kidney tissue using an oil objective. Without adaptive optics, the mismatch between immersion oil and sample leads to complete signal loss. Labels: Nephrin (red, Abberior STAR635P) and Podocin (green, AlexaFluor594).

Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.

Nuclear pore complexes (NUP) and tubulin imaged with confocal and EASY3D-STED microscopy.

Movie through a 2-color EASY3D RESCUE STED volume stack of a cleared adult kidney sample of a rat. Shown is a movie of an EASY3D and RESCUE volume stack of a renal corpuscle showing Nephrin (red, abberior STAR 635P) structures inbetween the Podocin slits (green, AlexaFluor594).

Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.

Surface Rendering of 2-color EASY3D volume of a cleared adult kidney sample of a rat. Shown is a reconstruction of an EASY3D volume stack of a renal corpuscle showing Nephrin (red, abberior STAR 635P) structures inbetween the Podocin slits (green, AlexaFluor594).

Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.

Tubulin imaged with EASY3D-STED vs confocal microscopy.

Two-color EASY3D-STED image of tubulin and giantin versus its confocal low-resolution counterpart.

DYMIN in living fibroplast cell. Note that 117 consecutive STED images have been recorded without fluorescence signal loss. Label: SiR-Tubulin.