Confocal stitched image of a spheroid stained for nuclear pores (magenta), actin cytoskeleton (blue), and mitochondria (orange).

NIH-313 spheroid, fixed with 4% PFA, kindly provided by ibidi and prepared on µ-Slide VI 0.4 µ-Pattern ibiTreat.

PFA-fixed mammalian cell stained by using Smart Secondaries^® from NanoTag Biotechnologies. Stained structures are intermediate filaments (cyan, abberior STAR 460L), mitochondria (green, abberior STAR GREEN), golgi (orange, abberior STAR ORANGE) and nuclear pore complex (magenta, abberior STAR RED).

Mitochondria labeled for the outer membrane protein TOMM20, imaged in confocal mode and with the MINFLUX module of the MIRAVA POLYSCOPE.

2D MINFLUX image of nuclear pore complex subunits, imaged in fixed mammalian cells expressing GFP-tagged NUP96 stained with abberior DNA-PAINT 660. The molecular resolution of the MINFLUX module of the MIRAVA POLYSCOPE allows visualizing the shape and arrangement of individual subunits of the nuclear pore complex.

Imaging across scales from diffraction-limited to molecular resolution: ribbon synapses in fixed mouse retina tissue stained for VAMP1 (abberior STAR RED) and CtBP2 (abberior STAR ORANGE) (confocal, MATRIX, STED) or for Bassoon (MINFLUX).

Sample courtesy: Arlene Hirano, PhD, University of California Los Angeles, Los Angeles, CA, USA (confocal, MATRIX, STED), and by Dr. Chad Grabner and Prof. Dr. Tobias Moser, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany (MINFLUX).

Video of mitochondria in living U2OS cells imaged for 1.5 hours with STED and deconvolved with TRUESHARP image boosting. Mitochondria were visualized with abberior LIVE SiR HaloX  staining the outer mitochondrial membrane marker TOMM20. FLEXPOSURE adaptive illumination was used to reduce the light burden on the sample.

A confocal image compared to a MATRIX + TRUESHARP STED image of nuclear pore complexes in mammalian cells stained for NUP96 with abberior STAR RED.

Living HeLa cells stained with the mitochondrial membrane marker abberior LIVE ORANGE mito, visualizing both outer and inner membranes. Confocal and STED images where deconvolved with TRUESHARP image boosting.

Confocal and STED image of a meiotic cell of Chinese spring wheat stained for two synaptonemal complex components.

Sample courtesy: Sepsi Adél, HUN-REN, Centre for Agricultural Research, Martonvásár, Hungary.

STED image of the golgi apparatus in a fixed mammalian cell, recorded with MATRIX array detection and deconvolved with TRUESHARP image boosting. Stained are the golgi proteins GM130 (cyan, abberior STAR RED) and giantin (orange, abberior STAR ORANGE).

Two-color 3D MINFLUX movie revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies) coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.

2D MINFLUX nanoscopy of the nuclear pore complex subunits, labeled with abberior FLUX 647 conjugated to JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies). In contrast to confocal microscopy, 2D MINFLUX allows visualization of the shape and arrangement of individual nuclear pore complex subunits. Here, we reach localization precisions of _~ 2 nm in raw localization data.

Two-color 3D MINFLUX revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies) coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.

Paraffin section of gut biopsy stained for Ki67 (abberior STAR ORANGE), Muc2 (abberior STAR RED), and DAPI.

xz section of a stage 17 Drosophila embryo stained for chitin (abberior LIVE 610, green) and DNA (abberior LIVE 550, cyan).

RAYSHAPE preserves resolution and brightness over the whole sample depth of about 200 µm by dynamically redirecting aberrated light to the right places.

In comparison, mechanical optics using a correction collar can only correct a limited z-range of approximately 20 µm

Drosophila stage 12 embryo, imaged with RAYSHAPE, stained for tubulin with abberior STAR RED and for DNA with abberior LIVE 550.

Deep tissue imaging with RAYSHAPE of a stage 17 Drosophila embryo stained for chitin with abberior LIVE 610.

Clear and detailed 3D rendering of trachea imaged with RAYSHAPE and MATRIX STED. Chitin was stained with abberior LIVE 610.

3D STED xz section of a Drosophila embryo trachea, imaged with RAYSHAPE and without abberation correction, depth 15 µm. Chitin was stained with abberior LIVE 610.

abberior STAR RED was coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch and used to label tubulin in fixed mammalian cells via indirect immunofluorescence. The STED image was acquired with the STEDYCON system.

Proteins of the nuclear pore complex and the golgi apparatus were stained by indirect immunofluorescence using abberior STAR RED (nuclear pore complex, magenta) and abberior STAR 580 (golgi, green) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.

Vimentin and tubulin were stained by indirect immunofluorescence using abberior STAR RED (vimentin, orange) and abberior STAR 580 (tubulin, cyan) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.

Indirect immunofluorescence staining with abberior STAR dyes coupled to polyclonal anti-mouse secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch shows excellent results in 3-color STED imaging. Staining was performed according to the premix & stain protocol. Vimentin (abberior STAR RED, green), tubulin (abberior STAR 580, magenta) and dsDNA (abberior STAR 460L, blue) were labeled in fixed mammalian cells and visualized with the STEDYCON.

2-color STED image of a living cell expressing actin K118TAG incorporating TCO*A labeled with abberior LIVE 590 click (green). Plasma membrane is highlighted with abberior STAR RED membrane (magenta).

2-color STED image of a living cell expressing actin^K118TAG incorporating TCO*A labeled with abberior LIVE 550 click (cyan). Tubulin filaments were stained with our direct probe abberior LIVE 610 tubulin (yellow).

Perfect 3-color combination for STED @ 775 nm. abberior STAR RED NUP98 (red) and STAR ORANGE vimentin (cyan) complemented by the new STAR 460L tubulin (yellow). Cultured mammalian cells were fixed and labeled by indirect immunofluorescence.

We are happy to introduce the first commercial long Stokes-shift dye for live-cell imaging applications. The NEW abberior LIVE 460L makes 3-color live-cell STED at 775 nm possible!

Live-cell STED image of a cell expressing a SNAP-tag® in an outer mitochondrial membrane protein visualized by our abberior LIVE 460L SNAP (orange). Tubulin filaments are highlighted with LIVE 610 (cyan) and the DNA with LIVE 560 DNA (red).

STED time-lapse of living mammalian cell stained with abberior STAR ORANGE membrane.

Two color live-cell STED and confocal image of a mammalian cultured cell stained with abberior STAR RED membrane (orange) and abberior LIVE 590 actin (cyan).

Comparison of STED and confocal of a living mammalian cell stained with abberior STAR ORANGE membrane.

Comparison of STED and confocal of a living mammalian cell stained with abberior STAR 488 membrane. Images were acquired with our FACILITY and STED @ 595 nm.

Confocal time-lapse of living mammalian cell stained with abberior STAR 488 membrane.

abberior CAGE 552 is a caged dye which is initially nonfluorescent and colorless substance which, upon photolysis with UV light, readily transforms irreversibly into a red and highly fluorescent dye.

The cytoskeleton is visible after UV induced uncaging of abberior CAGE 552.

3D MINFLUX image of the mitochondrial import receptor Tom20 labeled with abberior FLUX 647 in fixed mammalian cells using indirect immunolabeling. Please note that Tom20 is only localized at the mitochondrial surface. The axial coordinate is color-coded.

Two-color MINFLUX revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cell labeled with indirect immunofluorescence using secondary antibodies coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.

2D MINFLUX nanoscopy of the nuclear pore complex subunits. Fixed mammalian cells expressing SNAP-tag® NUP96 were labeled with abberior FLUX 647 SNAP. In contrast to confocal microscopy, 2D MINFLUX allows to visualize the shape and arrangement of individual subunits of the nuclear pore complex.

2D MINFLUX imaging of the peroxisomal membrane protein PMP70 labeled with abberior FLUX 640 in fixed mammalian cells using indirect immunofluorescence.

Composite of three color live-cell image of an adherent mammalian cell. This living cell was directly labelled with abberior LIVE 510 actin (blue/green), LIVE 560 DNA (gray) and LIVE 610 tubulin. This image was acquired with a STEDYCON microscope.

Three color live-cell STED at 775 nm: living cell labelled with abberior LIVE 460L (ER, green), LIVE 560 tubulin (magenta) and LIVE 610 actin (red).

Confocal and STED time lapse of a living cell expressing SNAP-tag® fusion protein fusion protein in the endoplasmic reticulum lumen with SNAP and C-terminal tetrapeptide KDEL. SNAP-KDEL was labelled with abberior LIVE 610 SNAP ligand (benzylguanine).

The SNAP-KDEL plasmid was a gift from Francesca Bottanelli, FU Berlin.

Live cell time lapse of abberior LIVE 590 DNA probe in cultured mammalian cells.

The use of very low concentrations of our abberior LIVE dyes reduces toxicity and allows long-term imaging of dynamic processes.

Image was acquired with the FACILITY microscope.

Two color live-cell confocal and STED image of a mammalian cell expressing a SNAP-tag® OMP25 fusion protein decoration the outer membrane of mitochondria. OMP25 is visualized by our new abberior LIVE 610 SNAP ligand (orange). Tubulin filaments are highlighted with abberior LIVE 550 tubulin (cyan).

The SNAP-OMP25 plasmid was a gift from Francesca Bottanelli, FU Berlin.

Two color live-cell confocal and STED image of a mammalian cell expressing a SNAP-tag® OMP25 fusion protein decoration the outer membrane of mitochondria. OMP25 is visualized by our new abberior LIVE 610 SNAP ligand (orange). Tubulin filaments are highlighted with abberior LIVE 550 tubulin (cyan).

The SNAP-OMP25 plasmid was a gift from Francesca Bottanelli, FU Berlin.

Cultured mammalian cell immunostained for an inner and outer mitochondrial membrane marker. Outer membrane is highlighted with abberior STAR RED (red) and the inner membrane with abberior STAR ORANGE (gray).

STED and confocal images were acquired with abberior's FACILITY microscope.

Three color STED and confocal image of a mammalian cultured cell immunostained for a nuclear pore protein in green and two golgi apparatus markers in red and blue.

abberior STAR RED highlights the golgi apparatus protein giantin (red) and abberior STAR ORANGE visualizes the cis-golgi protein GM130 (blue). NUP98 proteins were stained with abberior STAR GREEN (green).

Three color confocal image of human fibroblast immunostained with abberior STAR 580 for vimentin (green) and with abberior STAR RED for tubulin (red).

DAPI was added to highlight the nucleus (blue).

Four color confocal image of mammalian cultured cells. Peroxisomes were immunostained with abberior STAR RED (magenta) and nuclear pore proteins with abberior STAR 580 (green). F-actin fibers were highlighted with abberior STAR 488 phalloidin (gray).

The nucleus was visualized with DAPI (blue).

MINFLUX 3D nanoscopy of nuclear pore complex subunits. 3D MINFLUX facilitates 3D imaging at molecular scales. In the axial direction localization, we reach precisions of approx. 2.5 nm in raw localization data. Labeling of NUP96-SNAP was performed using SNAP-Alexa Fluor 647.