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MIRAVA POLYSCOPE – All in one and on for all: the perfect image
Science beyond Barriers

abberior instruments

Cell Biology

2021
Cells

Influence of subcellular localization and functional state on protein turnover

Authors:

Yousefi, R., Jevdokimenko, K., Kluever, V., Pacheu-Grau, D., & Fornasiero, E. F.

Keywords:

protein stability, optical analysis of protein turnover, SNAP-tag, pulse-chase, Rab5a

Abstract:

Protein homeostasis is an equilibrium of paramount importance that maintains cellular performance by preserving an efficient proteome. This equilibrium avoids the accumulation of potentially toxic proteins, which could lead to cellular stress and death. While the regulators of proteostasis are the machineries controlling protein production, folding and degradation, several other factors can influence this process. Here, we have considered two factors influencing protein turnover: the subcellular localization of a protein and its functional state. For this purpose, we used an imaging approach based on the pulse-labeling of 17 representative SNAP-tag constructs for measuring protein lifetimes. With this approach, we obtained precise measurements of protein turnover rates in several subcellular compartments. We also tested a selection of mutants modulating the function of three extensively studied proteins, the Ca2+ sensor calmodulin, the small GTPase Rab5a and the brain creatine kinase (CKB). Finally, we followed up on the increased lifetime observed for the constitutively active Rab5a (Q79L), and we found that its stabilization correlates with enlarged endosomes and increased interaction with membranes. Overall, our data reveal that both changes in protein localization and functional state are key modulators of protein turnover, and protein lifetime fluctuations can be considered to infer changes in cellular behavior.

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Superresolution & Confocal Systems

  • Overview
  • MINFLUX
  • MIRAVA POLYSCOPE
  • INFINITY
  • FACILITY
  • STEDYCON
  • Software Overview
  • LiGHTBOX
  • STEDYCON smart control
  • iMSPECTOR

Superresolution & Confocal Modules

  • Overview
  • MINFLUX Module
  • MATRIX Detector
  • TIMEBOW Imaging
  • FLEXPOSURE Illumination
  • RAYSHAPE Mirror
  • TRUESHARP Deconvolution
  • EASY3D
  • RAINBOW Detection
  • STED Lasers
  • Autoalignment
  • Autofocus
  • Excitation Lasers
  • Accessories
  • Custom Solutions

Dyes & Labels

  • Overview
  • abberior STAR
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  • abberior FLUX
  • abberior CAGE
  • abberior Supplies

Shop

  • Dyes by Product Name
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  • Dyes by Label
  • Mounting Medium
  • Cells and Nanoparticles

Applications

  • Overview
  • Biophysics
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  • Live Cell Imaging
  • Material Science
  • Membrane biology
  • Microbiology
  • Neurobiology
  • Physiology
  • Plant Science
  • Virology
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