Fluorescent rhodamines and fluorogenic carbopyronines for super‐resolution STED microscopy in living cells

Authors:

Butkevich, A. N., Mitronova, G. Y., Sidenstein, S. C., Klocke, J. L., Kamin, D., Meineke, D. N., ... & Hell, S. W.

Keywords:

carbopyronines, fluorescence, living cells, optical microscopy, rhodamines

Abstract:

A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500–630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye–ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure–property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40–60 nm.

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