MINFLUX module
Microscopy at the molecular level, straightforward and reliable – that is abberior’s MINFLUX module for the MIRAVA POLYSCOPE. Localize individual fluorophores in your sample with a precision of down to 3.2 nm to uncover previously unavailable information at the limits of light microscopy. And thanks to its seamless integration into our sleek LiGHTBOX software, setting up and controlling your MINFLUX measurement is a matter of just a few clicks.
single-digit nanometer resolution for MIRAVA POLYSCOPE
MINFLUX Module
Single-digit nanometer resolution with the MINFLUX module for our MIRAVA POLYSCOPE
MATRIX Detector
Many eyes see more than one. The MATRIX detector drastically improves signal-to-background ratio, resolution, and dynamic range.
TIMEBOW Imaging
TIMEBOW lifetime imaging for stunning results at confocal and STED super-resolution.
FLEXPOSURE Illumination
Brings down the light dose on your sample and lables dramatically. Key ingredient for volume and live-cell superresolution.
RAYSHAPE Mirror
Dynamic aberration correction with a deformable mirror over about 200 µm z-range. 140 digital actuators adjust the mirror surface within milliseconds.
Custom Solutions
We offer solutions for even the most challenging applications. Everything that can be done, we will do.
Description
Imaging across scales from diffraction-limited to molecular resolution: ribbon synapses in fixed mouse retina tissue stained for VAMP1 (abberior STAR RED) and CtBP2 (abberior STAR ORANGE) (confocal, MATRIX, STED) or for Bassoon (MINFLUX).
Sample courtesy: Arlene Hirano, PhD, University of California Los Angeles, Los Angeles, CA, USA (confocal, MATRIX, STED), and by Dr. Chad Grabner and Prof. Dr. Tobias Moser, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany (MINFLUX).
Modules:
Description
2D MINFLUX image of nuclear pore complex subunits, imaged in fixed mammalian cells expressing GFP-tagged NUP96 stained with abberior DNA-PAINT 660. The molecular resolution of the MINFLUX module of the MIRAVA POLYSCOPE allows visualizing the shape and arrangement of individual subunits of the nuclear pore complex.
Modules:
Description
Mitochondria labeled for the outer membrane protein TOMM20, imaged in confocal mode and with the MINFLUX module of the MIRAVA POLYSCOPE.
Modules:
Description
Dendrite with spines of a neuron grown on a coverslip, stained for βII spectrin and imaged in confocal mode and with the MINFLUX module of the MIRAVA POLYSCOPE.
Modules:
Description
Imaging across scales from diffraction-limited to molecular resolution: ribbon synapses in fixed mouse retina tissue stained for VAMP1 (abberior STAR RED) and CtBP2 (abberior STAR ORANGE) (confocal, MATRIX, STED) or for Bassoon (MINFLUX).
Sample courtesy: Arlene Hirano, PhD, University of California Los Angeles, Los Angeles, CA, USA (confocal, MATRIX, STED), and by Dr. Chad Grabner and Prof. Dr. Tobias Moser, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany (MINFLUX).
Modules:
FAQ Video 45
Is it easy to achieve nanometer resolution with the MINFLUX module in the MIRAVA POLYSCOPE?
MINFLUX module
single-digit nanometer resolution made easier than ever
Microscopy at the molecular level, straightforward and reliable – that is imaging with abberior’s MINFLUX module for our MIRAVA POLYSCOPE. Localize individual fluorophores in your sample with a precision of down to 3.2 nm to uncover previously unavailable information at the limits of light microscopy. And thanks to its seamless integration into our sleek LiGHTBOX software, setting up and controlling your MINFLUX measurement is a matter of just a few clicks.
Picture 1
LiGHTBOX software screenshot of a confocal overview image with the stabilization reference …
Picture 2
… a confocal image in the MINFLUX tab view …
Picture 3
… MINFLUX measurement with voxel view turned on, perfectly stabilized …
Picture 4
… for 1.5 minutes …
Picture 5
… for 10 minutes … or as long as it takes to get the most out of your sample!
MINFLUX module
simple protocols with superior dyes
From sample preparation to imaging and analysis, MINFLUX uses simple, well established workflows. Labeling works as with any other type of single molecule localization microscopy.
And since abberior always takes a holistic approach, we offer FLUX dyes that are designed for superior performance in MINFLUX imaging applications. They combine exceptional photostability, very high brightness, and low background labeling with photoswitching kinetics optimized for MINFLUX imaging. Conjugates of our dyes show excellent solubility in aqueous buffers and allow background-free labeling in cells or tissue.
MINFLUX module
minima-seeking, not maxima-bleaching
While other single molecule localization techniques require huge numbers of photons to achieve maximum resolution, MINFLUX localizes fluorophores by finding out where they do not emit when excited with a hollow beam – minima-seeking, not maxima-bleaching. It thus achieves nanometer-scale resolution that was previously achievable only with electron microscopes, but with the gentler touch of light, and without pushing the photon budget.
MINFLUX module
robust hardware meets intuitive software
The MINFLUX hardware yields unmatched robustness and optical quality. Movable parts are reduced to the absolute minimum to increase stability and reliability, and the stage corrects even subtle, sub-nanometer drifts caused by environmental changes (e.g. ambient temperature), keeping your sample perfectly still for days.
And with the user-friendly LiGHTBOX software, preparing and controlling your MINFLUX measurement is incredibly simple, no matter whether you are a novice or expert.
Focus on your measurement and let MIRAVA do the MINFLUX magic.
MINFLUX technology, system and module
changing the game
Our MINFLUX technology allows 3-dimensional localization precision of 2 to 3 nm in biological samples on fields of view of several tens of microns. With MINFLUX, molecular movements can be tracked with frequencies of up to 10 kHz, pinpointing the location of the tracked molecule every 100 µs.
Our MINFLUX system offers unprecedented spatial and temporal resolution in 3D, with two colors. It is 100x sharper than a confocal microscope and 100x faster than a camera.
Our MINFLUX module, that is seamlessly integrated into MIRAVA POLYSCOPE, allows single-color imaging with resolutions down to 3.2 nm in 2D and is 100 % as easy as any fluorescent microscope.
- Localization of individual fluorophores with a precision of down to 3.2 nm
- Perfectly integrated into the LiGHTBOX software for straightforward and fail-safe operation
- Easily upgrade your existing MIRAVA POLYSCOPE at any time and on site
abberior’s MINFLUX module offers single-digit nanometer resolution, easier than ever
Demo request MIRAVA POLYSCOPE
Seeing is believing, but you won’t believe your eyes! Get a MIRAVA demo and a first impression of our POLYSCOPE!
EVERGREEN special
EVERGREEN is abberior’s sustainable upgrade initiative for microscopes to elevate your research with a MIRAVA POLYSCOPE.
MIRAVA POLYSCOPE – Watch the premiere of the world’s only all-in-one solution for confocal, MATRIX, STED, and MINFLUX microscopy
abberior’s MIRAVA is the first true POLYSCOPE. Follow Stefan W. Hell, Matthias Reuss, Evelyn Garlick, and Martin Meschkat as they demonstrate all the features of our new microscope and take…
MINFLUX
The world’s most powerful fluorescence microscope MINFLUX, based on Stefan Hell’s invention, allows to resolve structures as small as a molecule in 3D.