RAYSHAPE dynamic aberration correction with a deformable mirror
The deeper you dive into a sample, the more severe the aberrations become, and the more you have to correct. In order to achieve zero aberrations throughout the sample, you therefore need to permanently adjust the amount of correction as focus depth changes.
abberior’s RAYSHAPE dynamic aberration correction solves this problem. Using adaptive optics with a deformable mirror, it puts diverted rays back on track, guaranteeing bright and crisp images from top to bottom and regardless of embedding medium, immersion medium, or specimen. RAYSHAPE aberration correction happens on-the-fly, with a deformable mirror, and is only available from abberior.
Dr. Matthias Reuss, Co-founder and head of R&D, explains RAYSHAPE and why you need it!
If you are interested in RAYSHAPE aberration correction with a deformable mirror, please contact us.
If you would like to test our perfectly fitting dyes for tissue imaging, you can order a free test kit consisting of our dyes abberior LIVE RED DNA (2 nmol) and abberior LIVE 590 Tubulin (2 nmol).
vs Correction collar
RAYSHAPE preserves resolution and brightness over the whole sample depth of about 200 µm by dynamically redirecting aberrated light to the right places. 140 digitally controlled actuators adjust our deformable mirror’s surface within milliseconds, making RAYSHAPE exceptionally precise and fast.
In comparison, mechanical optics using a correction collar can only correct a limited z-range of approximately 20 µm. In this example (xz-section of a stage 17 Drosophila embryo) a correction collar objective lens is set to correct aberrations at three different z-positions:
Correction collar close to the cover slip …
Correction collar at the middle of the z-range …
Correction collar at the end of the z-range. None of the positions is right for the whole sample and only a small z-range looks good.
RAYSHAPE deformable mirror with 140 actuators, tissue imaging has never felt better!