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MIRAVA POLYSCOPE – All in one and on for all: the perfect image
Science beyond Barriers

abberior dyes & labels

Technology

2013
Optical Engineering

Time-gated stimulated emission depletion nanoscopy

Authors:

Wang, Y., Kuang, C., Gu, Z., Xu, Y., Li, S., Hao, X., & Liu, X.

Abstract:

We have designed and built a time-gated continuous wave stimulated emission depletion (CW-STED) nanoscopy to visualize microstructures beyond the diffraction limit. An off-line time-gating detection was performed with the help of time-correlated single-photon counting technique. Experimental results showed that before time-gating, the resolution of our system was about 75 nm with a depletion beam (592 nm) power of 200 mW. By using the off-line time-gating detection, the resolution was further improved to 38 nm. Biological samples were also used to test the performance of our time-gated CW-STED, and a resolution of 70 nm was achieved with a depletion beam (592 nm) power of 85 mW. Detailed principles of time-gated CW-STED were discussed in the text. The time-gated STED provides a better resolution with finite laser power.

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Superresolution & Confocal Systems

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Superresolution & Confocal Modules

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  • MINFLUX Module
  • MATRIX Detector
  • TIMEBOW Imaging
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  • RAYSHAPE Mirror
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