Products

Superresolution & Confocal Systems

  • Overview
  • MINFLUX
  • INFINITY
  • FACILITY
  • STEDYCON

Superresolution & Confocal Modules

  • Overview
  • MATRIX Detector
  • TIMEBOW Imaging
  • Adaptive Illumination
  • Adaptive Optics
  • EASY3D
  • RAINBOW Detection
  • STED Lasers
  • Autoalignment
  • Autofocus
  • Excitation Lasers
  • Accessories
  • Custom Solutions

Dyes & Labels

  • Overview
  • abberior STAR
  • abberior LIVE
  • abberior FLUX
  • abberior CAGE
  • abberior Supplies

Shop

  • Dyes by Product Name
  • Dyes by Technique
  • Dyes by Function
  • Microscopy Supplies

Applications

  • Overview
  • Live Cell Imaging
  • Cell Biology
  • Neurobiology
  • Virology
  • Microbiology
  • Zoology
  • Plant Sciences
  • Biophysics
  • Physiology
  • Membrane Biology
  • Material Science
  • All-purpose
  • Tailor-Made

Company

  • People
  • Founders
  • Mission
  • Career
  • Locations

News & Events

  • News
  • Events
  • Webinars
  • Past Events

Expertise

  • Knowledge Base
  • FAQ Videos
  • FAQ
  • Publications
  • White Papers
  • Sample Gallery
  • Microscopy Tutorials
  • Webinar Recordings
  • Protocols
  • Support
  • Contact
  • Shop
  • Search
  • EN
    中文
    Language
@abberior.rocks
MENU Contact
ready to useReady to use MINFLUX 3D
Science beyond Barriers

abberior instruments

2019
Nature communications

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

Authors:

Götzke, H., Kilisch, M., Martínez-Carranza, M., Sograte-Idrissi, S., Rajavel, A., Schlichthaerle, T., ... & Frey, S.

Keywords:

Assay systems, Fluorescence imaging, Preclinical research, Protein purification, Super-resolution microscopy

Abstract:

Specialized epitope tags are widely used for detecting, manipulating or purifying proteins, but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed epitope tag that serves a remarkably broad spectrum of applications in life sciences while outperforming established tags like the HA-, FLAG®- or myc-tag. The ALFA-tag forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts. We characterize a nanobody (NbALFA) binding ALFA-tagged proteins from native or fixed specimen with low picomolar affinity. It is ideally suited for super-resolution microscopy, immunoprecipitations and Western blotting, and also allows in vivo detection of proteins. We show the crystal structure of the complex that enabled us to design a nanobody mutant (NbALFAPE) that permits efficient one-step purifications of native ALFA-tagged proteins, complexes and even entire living cells using peptide elution under physiological conditions.

< Back to publications
Full article >
linkedin facebook twitter Instagram

World+49 551 9995 4010USA+1 301 661 0078

© 2023 abberior

Superresolution & Confocal Systems

  • Overview
  • MINFLUX
  • INFINITY
  • FACILITY
  • STEDYCON

Superresolution & Confocal Modules

  • Overview
  • MATRIX Detector
  • TIMEBOW Imaging
  • Adaptive Illumination
  • Adaptive Optics
  • EASY3D
  • RAINBOW Detection
  • STED Lasers
  • Autoalignment
  • Autofocus
  • Excitation Lasers
  • Accessories
  • Custom Solutions

Dyes & Labels

  • Overview
  • abberior STAR
  • abberior LIVE
  • abberior FLUX
  • abberior CAGE
  • abberior Supplies

Shop

  • Dyes by Product Name
  • Dyes by Technique
  • Dyes by Function
  • Microscopy Supplies

Applications

  • Overview
  • Live Cell Imaging
  • Cell Biology
  • Neurobiology
  • Virology
  • Microbiology
  • Zoology
  • Plant Sciences
  • Biophysics
  • Physiology
  • Membrane Biology
  • Material Science
  • All-purpose
  • Tailor-Made

Company

  • People
  • Founders
  • Mission
  • Career
  • Locations

News & Events

  • News
  • Events
  • Webinars
  • Past Events

Expertise

  • Knowledge Base
  • FAQ Videos
  • FAQ
  • Publications
  • White Papers
  • Sample Gallery
  • Microscopy Tutorials
  • Webinar Recordings
  • Protocols
abberior instruments GmbH:
  • Imprint
  • Privacy Policy
  • Terms of Sale
abberior GmbH:
  • Imprint
  • Privacy Policy
  • Terms of Sale
Abberior Instruments America LLC:
  • Privacy Policy
  • Terms of use USA
  • contact
  • manuals
  • service
  • shop

Cookie settings

Please select whether this page may use cookies. Technically necessary cookies are always set. You can also find further information in our data protection statement.

These cookies are required to enable the basic functions of this website, we set a cookie accordingly. This saves your individual consent to the use of technically necessary cookies. Our website only uses technically necessary cookies.


Privacy PolicyImprint