abberior dyes & labels
Terminal deoxynucleotidyl transferase mediated production of labeled probes for single-molecule FISH or RNA capture
Gaspar, I., Wippich, F., & Ephrussi, A.
Terminal deoxynucleotidyl transferase, Labelled terminator nucleotide, Probe production, Single molecule FISH (smFISH), RNA capture, RNA affinity purification (RAP)
Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using basic laboratory equipment to convert arrays of PCR oligos into smFISH and RAP probesets in a quantitative, cost-efficient and flexible way.