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Live Cell Imaging, Membrane Biology

2018
In Clathrin-Mediated Endocytosis (pp. 189-195). Humana Press

Stimulated emission depletion (STED) imaging of clathrin-mediated endocytosis in living cells

Authors:

Bottanelli, F., & Schroeder, L.

Abstract:

The recent development of probes and labeling strategies for multicolor super-resolution imaging in living cells allows cell biologists to follow cellular processes with unprecedented details. Here we describe how to image endocytic events at the plasma membrane of living cells using commercial (Leica, Abberior Instruments) or custom built STED microscopes.

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