Products

Superresolution & Confocal Systems

  • Overview
  • MINFLUX
  • INFINITY
  • FACILITY
  • STEDYCON

Superresolution & Confocal Modules

  • Overview
  • MATRIX Detector
  • TIMEBOW Imaging
  • Adaptive Illumination
  • Adaptive Optics
  • EASY3D
  • RAINBOW Detection
  • STED Lasers
  • Autoalignment
  • Autofocus
  • Excitation Lasers
  • Accessories
  • Custom Solutions

Dyes & Labels

  • Overview
  • abberior STAR
  • abberior LIVE
  • abberior FLUX
  • abberior CAGE
  • abberior Supplies

Shop

  • Dyes by Product Name
  • Dyes by Technique
  • Dyes by Function
  • Microscopy Supplies

Applications

  • Overview
  • Live Cell Imaging
  • Cell Biology
  • Neurobiology
  • Virology
  • Microbiology
  • Zoology
  • Plant Sciences
  • Biophysics
  • Physiology
  • Membrane Biology
  • Material Science
  • All-purpose
  • Tailor-Made

Company

  • People
  • Founders
  • Mission
  • Career
  • Locations

News & Events

  • News
  • Events
  • Webinars
  • Past Events

Expertise

  • Knowledge Base
  • FAQ Videos
  • FAQ
  • Publications
  • White Papers
  • Sample Gallery
  • Microscopy Tutorials
  • Webinar Recordings
  • Protocols
  • Support
  • Contact
  • Shop
  • Search
  • EN
    中文
    Language
@abberior.rocks
MENU Contact
ready to useReady to use MINFLUX 3D
Science beyond Barriers

abberior dyes & labels

2021
Development

Single-molecule tracking technologies for quantifying the dynamics of gene regulation in cells, tissue and embryos

Authors:

Boka, A. P., Mukherjee, A., & Mir, M.

Keywords:

Fluorescence, Gene regulation, Light-sheet, Single-molecule microscopy, Single-molecule tracking, Transcription

Abstract:

For decades, we have relied on population and time-averaged snapshots of dynamic molecular scale events to understand how genes are regulated during development and beyond. The advent of techniques to observe single-molecule kinetics in increasingly endogenous contexts, progressing from in vitro studies to living embryos, has revealed how much we have missed. Here, we provide an accessible overview of the rapidly expanding family of technologies for single-molecule tracking (SMT), with the goal of enabling the reader to critically analyse single-molecule studies, as well as to inspire the application of SMT to their own work. We start by overviewing the basics of and motivation for SMT experiments, and the trade-offs involved when optimizing parameters. We then cover key technologies, including fluorescent labelling, excitation and detection optics, localization and tracking algorithms, and data analysis. Finally, we provide a summary of selected recent applications of SMT to study the dynamics of gene regulation.

< Back to publications
Full article >
linkedin facebook twitter Instagram

World+49 551 9995 4010USA+1 301 661 0078

© 2023 abberior

Superresolution & Confocal Systems

  • Overview
  • MINFLUX
  • INFINITY
  • FACILITY
  • STEDYCON

Superresolution & Confocal Modules

  • Overview
  • MATRIX Detector
  • TIMEBOW Imaging
  • Adaptive Illumination
  • Adaptive Optics
  • EASY3D
  • RAINBOW Detection
  • STED Lasers
  • Autoalignment
  • Autofocus
  • Excitation Lasers
  • Accessories
  • Custom Solutions

Dyes & Labels

  • Overview
  • abberior STAR
  • abberior LIVE
  • abberior FLUX
  • abberior CAGE
  • abberior Supplies

Shop

  • Dyes by Product Name
  • Dyes by Technique
  • Dyes by Function
  • Microscopy Supplies

Applications

  • Overview
  • Live Cell Imaging
  • Cell Biology
  • Neurobiology
  • Virology
  • Microbiology
  • Zoology
  • Plant Sciences
  • Biophysics
  • Physiology
  • Membrane Biology
  • Material Science
  • All-purpose
  • Tailor-Made

Company

  • People
  • Founders
  • Mission
  • Career
  • Locations

News & Events

  • News
  • Events
  • Webinars
  • Past Events

Expertise

  • Knowledge Base
  • FAQ Videos
  • FAQ
  • Publications
  • White Papers
  • Sample Gallery
  • Microscopy Tutorials
  • Webinar Recordings
  • Protocols
abberior instruments GmbH:
  • Imprint
  • Privacy Policy
  • Terms of Sale
abberior GmbH:
  • Imprint
  • Privacy Policy
  • Terms of Sale
Abberior Instruments America LLC:
  • Privacy Policy
  • Terms of use USA
  • contact
  • manuals
  • service
  • shop

Cookie settings

Please select whether this page may use cookies. Technically necessary cookies are always set. You can also find further information in our data protection statement.

These cookies are required to enable the basic functions of this website, we set a cookie accordingly. This saves your individual consent to the use of technically necessary cookies. Our website only uses technically necessary cookies.


Privacy PolicyImprint