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Science beyond Barriers

abberior dyes & labels

2021
Biophysics Reports

Recent progress on single-molecule localization microscopy

Authors:

Lusheng, G., & Wei, J.

Keywords:

Single-molecule localization microscopy, Super-resolution imaging, Centroid fitting, Localization precision

Abstract:

Super-resolution imaging based on single-molecule localization has been developed for more than a decade. These techniques can break through diffraction limit of fluorescent microscopy and initially improve the resolution by an order of magnitude to ~20 nm, by introducing photoactivatable/photoswitching probes and centroid fitting method. As the demand of biological research, the localization precision of single-molecules was further improved by several state-of-the-art methods in the past several years. This review focuses on the latest developed techniques which have greatly improved the performance of single-molecule localization microscopy, from measurement principle to hardware design. These methods are essential for the study of nanostructures and biomacromolecule dynamics inside of cells.

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