Photochromic rhodamines provide nanoscopy with optical sectioning

Authors:

Fölling, J., Belov, V., Kunetsky, R., Medda, R., Schönle, A., Egner, A., ... & Hell, S. E.

Keywords:

photochromism, photoswitchable compounds

Abstract:

Exciting developments: Switching individual photochromic and fluorescent rhodamine amides enables 3D far-field optical microscopy with nanoscale resolution, excellent signal-to-noise ratio, and fast acquisition times. The rhodamine amides can be switched on using two photons, which enables 3D detailed imaging of thick and densely stained samples (such as 5-μm silica beads (see image) and living cells) to be constructed.

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