Products

Superresolution & Confocal Systems

  • Overview
  • MINFLUX
  • MIRAVA POLYSCOPE
  • INFINITY
  • FACILITY
  • STEDYCON
  • Software Overview
  • LiGHTBOX
  • STEDYCON smart control
  • iMSPECTOR

Superresolution & Confocal Modules

  • Overview
  • MINFLUX Module
  • MATRIX Detector
  • TIMEBOW Imaging
  • FLEXPOSURE Illumination
  • RAYSHAPE Mirror
  • TRUESHARP Deconvolution
  • EASY3D
  • RAINBOW Detection
  • STED Lasers
  • Autoalignment
  • Autofocus
  • Excitation Lasers
  • Accessories
  • Custom Solutions

Dyes & Labels

  • Overview
  • abberior STAR
  • abberior LIVE
  • abberior FLUX
  • abberior CAGE
  • abberior Supplies

Shop

  • Dyes by Product Name
  • Dyes by Technique
  • Dyes by Label
  • Mounting Medium
  • Cells and Nanoparticles

Applications

  • Overview
  • Biophysics
  • Cell Biology
  • Live Cell Imaging
  • Material Science
  • Membrane biology
  • Microbiology
  • Neurobiology
  • Physiology
  • Plant Science
  • Virology
  • Zoology

Company

  • People
  • Founders
  • Mission
  • Career
  • Vacancies
  • Locations

News & Events

  • News
  • Events
  • Webinars
  • Past Events

Expertise

  • Knowledge Base
  • FAQ Videos
  • FAQ
  • Publications
  • White Papers
  • Sample Gallery
  • Microscopy Tutorials
  • Webinar Recordings
  • Protocols
  • Support
  • Contact
  • Shop
  • Search
  • EN
    中文
    Language
@abberior.rocks
MENU Contact
MIRAVA POLYSCOPE – All in one and on for all: the perfect image
Science beyond Barriers

abberior instruments

2021
Journal of the American Chemical Society

Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy

Authors:

Butkevich, A. N., Weber, M., Cereceda Delgado, A. R., Ostersehlt, L. M., D’Este, E., & Hell, S. W.

Keywords:

Peptides and proteins, MINFLUX

Abstract:

We propose a series of fluorescent dyes with hydrophilic carbamate caging groups that undergo rapid photoactivation under UV (≤400 nm) irradiation but do not undergo spurious two-photon activation with high-intensity (visible or infrared) light of about twice the wavelength. The caged fluorescent dyes and labels derived therefrom display high water solubility and convert upon photoactivation into validated super-resolution and live-cell-compatible fluorophores. In combination with popular fluorescent markers, multiple (up to six)-color images can be obtained with stimulated emission depletion nanoscopy. Moreover, individual fluorophores can be localized with precision <3 nm (standard deviation) using MINSTED and MINFLUX techniques.

< Back to publications
Full article >
linkedin facebook bluesky twitter Instagram

World+49 551 9995 4010USA+1 301 661 0078

© 2025 abberior

Superresolution & Confocal Systems

  • Overview
  • MINFLUX
  • MIRAVA POLYSCOPE
  • INFINITY
  • FACILITY
  • STEDYCON
  • Software Overview
  • LiGHTBOX
  • STEDYCON smart control
  • iMSPECTOR

Superresolution & Confocal Modules

  • Overview
  • MINFLUX Module
  • MATRIX Detector
  • TIMEBOW Imaging
  • FLEXPOSURE Illumination
  • RAYSHAPE Mirror
  • TRUESHARP Deconvolution
  • EASY3D
  • RAINBOW Detection
  • STED Lasers
  • Autoalignment
  • Autofocus
  • Excitation Lasers
  • Accessories
  • Custom Solutions

Dyes & Labels

  • Overview
  • abberior STAR
  • abberior LIVE
  • abberior FLUX
  • abberior CAGE
  • abberior Supplies

Shop

  • Dyes by Product Name
  • Dyes by Technique
  • Dyes by Label
  • Mounting Medium
  • Cells and Nanoparticles

Applications

  • Overview
  • Biophysics
  • Cell Biology
  • Live Cell Imaging
  • Material Science
  • Membrane biology
  • Microbiology
  • Neurobiology
  • Physiology
  • Plant Science
  • Virology
  • Zoology

Company

  • People
  • Founders
  • Mission
  • Career
  • Vacancies
  • Locations

News & Events

  • News
  • Events
  • Webinars
  • Past Events

Expertise

  • Knowledge Base
  • FAQ Videos
  • FAQ
  • Publications
  • White Papers
  • Sample Gallery
  • Microscopy Tutorials
  • Webinar Recordings
  • Protocols
abberior instruments GmbH:
  • Imprint
  • Privacy Policy
  • Terms of Sale
abberior GmbH:
  • Imprint
  • Privacy Policy
  • Terms of Sale
Abberior Instruments America LLC:
  • Privacy Policy
  • Terms of use USA
  • contact
  • manuals
  • service
  • shop