Nanoscopy in a living mouse brain
Berning, S., Willig, K. I., Steffens, H., Dibaj, P., & Hell, S. W.
We demonstrated superresolution optical microscopy in a living higher animal. Stimulated emission depletion (STED) fluorescence nanoscopy reveals neurons in the cerebral cortex of a mouse with <70-nanometer resolution. Dendritic spines and their subtle changes can be observed at their relevant scales over extended periods of time.