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Science beyond Barriers

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Neurobiology

2012
Science

Nanoscopy in a living mouse brain

Authors:

Berning, S., Willig, K. I., Steffens, H., Dibaj, P., & Hell, S. W.

Abstract:

We demonstrated superresolution optical microscopy in a living higher animal. Stimulated emission depletion (STED) fluorescence nanoscopy reveals neurons in the cerebral cortex of a mouse with <70-nanometer resolution. Dendritic spines and their subtle changes can be observed at their relevant scales over extended periods of time.

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