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MIRAVA POLYSCOPE – All in one and on for all: the perfect image
Science beyond Barriers

abberior instruments

Membrane Biology

2023
bioRxiv

Multi-functional ARF1 compartments serve as a hub for short-range cargo transfer to endosomes

Authors:

Alexander Stockhammer, Petia Adarska, Vini Natalia, Anja Heuhsen, Antonia Klemt, Shelly Harel, Carmen Rodilla-Ramirez, Carissa Spalt, Ece Özsoy, Paula Leupold, Alica Grindel, Eleanor Fox, Joy Orezimena Mejedo, Dmytro Puchkov, Volker Haucke, Francesca Bottanelli

Keywords:

kiss-and-run; vesicle; membrane; membrane homeostasis; GTPase; ARF1; Adaptor Protein Complex; endosomes

Abstract:

Cellular membrane homeostasis is maintained via a tightly regulated membrane and cargo flow between organelles of the endocytic and secretory pathways. Adaptor protein complexes (APs), which are recruited to membranes by the small GTPase ARF1, facilitate cargo selection and incorporation into trafficking intermediates. According to the classical model, small vesicles would facilitate bi-directional long-range vesicular transport between the Golgi and endosomes. Here we revisit the intracellular organization of the vesicular sorting machinery using a combination of CRISPR-Cas9 gene editing, live-cell high temporal (fast-confocal) or spatial (stimulated emission depletion (STED)) microscopy as well as correlative light and electron microscopy. We characterize novel tubulo-vesicular ARF1 compartments that harbor clathrin and different APs, and coordinate Golgi export as well as endocytic recycling. Rather than long-range vesicle shuttling, we observe transient interactions of ARF1 compartments and recycling endosomes, with the sorting machinery localizing at the interface between the two membranes. Our findings suggest that endocytic and secretory cargo transfer from ARF1 compartments to endosomes may be mediated by a kiss-and-run mechanism rather than by fusion of transport vesicles.

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Superresolution & Confocal Systems

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Superresolution & Confocal Modules

  • Overview
  • MINFLUX Module
  • MATRIX Detector
  • TIMEBOW Imaging
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  • EASY3D
  • RAINBOW Detection
  • STED Lasers
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  • Excitation Lasers
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