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Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy
Chen, Y., Liu, W., Zhang, Z., Zheng, C., Huang, Y., Cao, R., ... & Liu, X.
Total internal reflection microscopy, Wide-field fluorescence microscopy
Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dynamics of mitochondria and microtubule architectures during cell migration.