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Science beyond Barriers

abberior dyes & labels

2018
Nature methods

Monitoring molecular jumps

Authors:

Strack, R.

Abstract:

The recently described MINFLUX nanoscopy method allows for high-resolution monitoring of single molecules with a small fraction of the photons needed for conventional super-resolution microscopy. Although the method was shown to work well for high-resolution imaging of DNA origami structures and for single-particle tracking with high spatiotemporal resolution, its benefits for the investigation of single-particle movements were not fully explored. Eilers et al. now show that MINFLUX can be used to monitor molecular movements of a few nanometers at rates that far surpass the spatiotemporal resolution of conventional approaches. They demonstrated their approach by studying the thermal fluctuation of fluorophore-labeled DNA strands, and achieved ~2-nanometer localization precision with measurements as short as 400 microseconds. These results highlight the power of this approach for studying the dynamics of single molecules.

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