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MIRAVA POLYSCOPE – All in one and on for all: the perfect image
Science beyond Barriers

abberior instruments

2018
Proceedings of the National Academy of Sciences

MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution

Authors:

Eilers, Y., Ta, H., Gwosch, K. C., Balzarotti, F., & Hell, S. W.

Keywords:

MINFLUX

Abstract:

Compared with localization schemes solely based on evaluating patterns of molecular emission, the recently introduced single-molecule localization concept called MINFLUX and the fluorescence nanoscopies derived from it require up to orders of magnitude fewer emissions to attain single-digit nanometer resolution. Here, we demonstrate that the lower number of required fluorescence photons enables MINFLUX to detect molecular movements of a few nanometers at a temporal sampling of well below 1 millisecond. Using fluorophores attached to thermally fluctuating DNA strands as model systems, we demonstrate that measurement times as short as 400 microseconds suffice to localize fluorescent molecules with ∼2-nm precision. Such performance is out of reach for popular camera-based localization by centroid calculation of emission diffraction patterns. Since theoretical limits have not been reached, our results show that emerging MINFLUX nanoscopy bears great potential for dissecting the motions of individual (macro)molecules at hitherto-unattained combinations of spatial and temporal resolution.

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Superresolution & Confocal Systems

  • Overview
  • MINFLUX
  • MIRAVA POLYSCOPE
  • INFINITY
  • FACILITY
  • STEDYCON
  • Software Overview
  • LiGHTBOX
  • STEDYCON smart control
  • iMSPECTOR

Superresolution & Confocal Modules

  • Overview
  • MINFLUX Module
  • MATRIX Detector
  • TIMEBOW Imaging
  • FLEXPOSURE Illumination
  • RAYSHAPE Mirror
  • TRUESHARP Deconvolution
  • EASY3D
  • RAINBOW Detection
  • STED Lasers
  • Autoalignment
  • Autofocus
  • Excitation Lasers
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Dyes & Labels

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  • abberior STAR
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  • abberior FLUX
  • abberior CAGE
  • abberior Supplies

Shop

  • Dyes by Product Name
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  • Dyes by Label
  • Mounting Medium
  • Cells and Nanoparticles

Applications

  • Overview
  • Biophysics
  • Cell Biology
  • Live Cell Imaging
  • Material Science
  • Membrane biology
  • Microbiology
  • Neurobiology
  • Physiology
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  • Virology
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