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MIRAVA POLYSCOPE – All in one and on for all: the perfect image
Science beyond Barriers

abberior instruments

Cell Biology, Physiology

2023
Journal of Cell Biology

Hepatocyte apical bulkheads provide a mechanical means to oppose bile pressure

Authors:

Maarten P Bebelman, Matthew J Bovyn, Carlotta M Mayer, Julien Delpierre, Ronald Naumann, Nuno P Martins, Alf Honigmann, Yannis Kalaidzidis, Pierre A Haas, Marino Zerial

Keywords:

Hepatocytes, Apicobasal polarity, tight junctions

Abstract:

Hepatocytes grow their apical surfaces anisotropically to generate a 3D network of bile canaliculi (BC). BC elongation is ensured by apical bulkheads, membrane extensions that traverse the lumen and connect juxtaposed hepatocytes. We hypothesize that apical bulkheads are mechanical elements that shape the BC lumen in liver development but also counteract elevated biliary pressure. Here, by resolving their structure using STED microscopy, we found that they are sealed by tight junction loops, connected by adherens junctions, and contain contractile actomyosin, characteristics of mechanical function. Apical bulkheads persist at high pressure upon microinjection of fluid into the BC lumen, and laser ablation demonstrated that they are under tension. A mechanical model based on ablation results revealed that apical bulkheads double the pressure BC can hold. Apical bulkhead frequency anticorrelates with BC connectivity during mouse liver development, consistent with predicted changes in biliary pressure. Our findings demonstrate that apical bulkheads are load-bearing mechanical elements that could protect the BC network against elevated pressure.

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Superresolution & Confocal Systems

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Superresolution & Confocal Modules

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  • MINFLUX Module
  • MATRIX Detector
  • TIMEBOW Imaging
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