Fluorescence Microscopy: A Field Guide for Biologists

Authors:

Swift, L. H., & Colarusso, P.

Keywords:

Digital image, Imaging workflow, Image acquisition, Lateral resolution, Nyquist sampling, Data management plan

Abstract:

Optical microscopy is a tool for observing objects, and features within objects, that are not visible to the unaided eye. In the life sciences, fluorescence microscopy has been widely adopted because it allows us to selectively observe molecules, organelles, and cells at multiple levels of organization. Fluorescence microscopy encompasses numerous techniques and applications that share a specialized technical language and concepts that can create barriers for researchers who are new to this area. Our goal is to meet the needs of researchers new to fluorescence microscopy, by introducing the essential concepts and mindset required to navigate and apply this powerful technology to the laboratory.

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