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RAYSHAPE – bye bye abarrations
Science beyond Barriers

abberior instruments

Technology

2022
bioRxiv

Exchangeable HaloTag Ligands (xHTLs) for multi-modal super-resolution fluorescence microscopy

Authors:

Kompa, J., Bruins, J., Glogger, M., Wilhelm, J., Frei, M. S., Tarnawski, M., ... & Johnsson, K.

Abstract:

We introduce exchangeable ligands for fluorescence labeling of HaloTag7 as an alternative to covalently bound probes. The exchangeable ligands open up new possibilities in imaging for a widely used labeling approach, including applications in points accumulation for imaging in nanoscale topography (PAINT), MINFLUX and live-cell, multi-frame stimulated emission depletion (STED) microscopy. We furthermore introduce orthogonal pairs of exchangeable ligands and HaloTags for dual-color PAINT and STED microscopy.

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