Exchangeable HaloTag Ligands (xHTLs) for multi-modal super-resolution fluorescence microscopy
Kompa, J., Bruins, J., Glogger, M., Wilhelm, J., Frei, M. S., Tarnawski, M., ... & Johnsson, K.
We introduce exchangeable ligands for fluorescence labeling of HaloTag7 as an alternative to covalently bound probes. The exchangeable ligands open up new possibilities in imaging for a widely used labeling approach, including applications in points accumulation for imaging in nanoscale topography (PAINT), MINFLUX and live-cell, multi-frame stimulated emission depletion (STED) microscopy. We furthermore introduce orthogonal pairs of exchangeable ligands and HaloTags for dual-color PAINT and STED microscopy.