abberior dyes & labels
Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser
Han, K. Y., & Ha, T.
Acoustooptic tunable filters, Fluorescent markers, Imaging systems, Laser sources, Photonic crystal fibers, Three dimensional microscopy
We describe a dual-color three-dimensional stimulated emission depletion (3D-STED) microscopy employing a single laser source with a repetition rate of 80 MHz. Multiple excitation pulses synchronized with a STED pulse were generated by a photonic crystal fiber, and the desired wavelengths were selected by an acousto-optic tunable filter with high spectral purity. Selective excitation at different wavelengths permits simultaneous imaging of two fluorescent markers at a nanoscale resolution in three dimensions.