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Science beyond Barriers

abberior instruments

Cell Biology

2023
bioRxiv

Deep learning enables fast, gentle STED microscopy

Authors:

Vahid Ebrahimi, Till Stephan, Jiah Kim, Pablo Carravilla, Christian Eggeling, Stefan Jakobs, Kyu Young Han

Keywords:

Mitochondria, STED Nanoscopy, Deep Learning

Abstract:

STED microscopy is widely used to image subcellular structures with super-resolution. Here, we report that denoising STED images with deep learning can mitigate photobleaching and photodamage by reducing the pixel dwell time by one or two orders of magnitude. Our method allows for efficient and robust restoration of noisy 2D and 3D STED images with multiple targets and facilitates long-term imaging of mitochondrial dynamics.

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