A protocol for single‐source dual‐pulse stimulated emission depletion setup with Bessel modulation

Authors:

Xu, X., Zhao, K., Ren, W., Wu, Z., Yu, W., Shao, C., ... & Xi, P.

Keywords:

Bessel modulation, detector saturation

Abstract:

STimulated Emission Depletion (STED) microscopy attains super-resolution in biological imaging beyond the diffraction limit. Here, we give a concise protocol to construct a dual-pulse STED setup with one super-continuum laser. Moreover, a flexible and dismountable Bessel modulation module is introduced for potential 2D-stack STED imaging. Experiments and notices are introduced in detail, with discussion on some important check-points for STED, such as detector saturation. Finally, the results validate the system working.

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