abberior dyes & labels
Microscopy Research and Technique
A protocol for single‐source dual‐pulse stimulated emission depletion setup with Bessel modulation
Xu, X., Zhao, K., Ren, W., Wu, Z., Yu, W., Shao, C., ... & Xi, P.
Bessel modulation, detector saturation
STimulated Emission Depletion (STED) microscopy attains super-resolution in biological imaging beyond the diffraction limit. Here, we give a concise protocol to construct a dual-pulse STED setup with one super-continuum laser. Moreover, a flexible and dismountable Bessel modulation module is introduced for potential 2D-stack STED imaging. Experiments and notices are introduced in detail, with discussion on some important check-points for STED, such as detector saturation. Finally, the results validate the system working.