NirFAP680: a highly bright and stable large Stokes shift near-infrared fluorogen-activating protein

Authors:

Zhengda Chen, Li Jiang, Xiaoyu Liu, Yajie Shi, Yang Liu, Ling Jiang, Jiaxin Li, Xinyi Huang, Siyu Zhou, Bingkun Bao, Bibi Zhang, Qiuning Lin, Qi Bian, Yuxin Yin, Fangting Zuo, Ni Su, Yun Tang, Yuzheng Zhao, Yi Yang, Xianjun Chen, Linyong Zhu

Keywords:

Near-infrared; fluorogen-activating protein; genetically encoded fluorescent tags

Abstract:

Near-infrared (NIR) genetically encoded fluorescent tags are superior for new imaging capabilities ranging from multiplexed imaging to deep-tissue imaging. Here, we describe the development of NirFAP680; NirFAP680 is an NIR fluorogen-activating protein (FAP) that consists of a newly designed NIR fluorogen termed HBMT and an engineered protein tag termed NirFAP. NirFAP680 has an order of magnitude greater cellular brightness and superior photostability compared to currently available NIR FAPs and fluorescent proteins in both single- and two-photon excitation and allows robust imaging of proteins in live cells and in vivo. Owing to its unique spectroscopic property of a >100 nm Stokes shift, NirFAP680 can also serve as a superb receptor for fluorescence or bioluminescence resonance energy transfer, allowing real-time monitoring of protein–protein interactions in live cells and sensitive bioluminescence imaging in vivo, respectively. Therefore, NirFAP680 will likely be useful for advanced biological imaging in live cells and in vivo.

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