CIP2A mediates mitotic recruitment of SLX4/MUS81/XPF to resolve replication stress-induced DNA lesions

Authors:

Lauren de Haan, Sietse J. Dijt, Alejandro García-López, Dan Ruan, Panagiotis Martzios, Femke J. Bakker, Marieke Everts, Harry Warner, Frank N. Mol, J. Ross Chapman, H. Rudolf de Boer, Bert van de Kooij, Pim J. Huis in ’t Veld, Rifka Vlijm, Marcel A. T. M. van Vugt

Keywords:

CIP2A; mitotic recruitment, DNA lesion; replication-stress; DNA replication; mitosis; chromosome segregation; CIP2A-TOPBP1 complex; SLX4/MUS81/XPF; SMX complex

Abstract:

Perturbed DNA replication can lead to incompletely replicated DNA when cells enter mitosis and can interfere with chromosome segregation. Cells therefore require mechanisms to resolve these lesions during mitosis. The CIP2A-TOPBP1 complex is described to tether fragmented DNA molecules during mitosis. Whether CIP2A also functions in processing of incompletely replicated DNA remained unclear. We show that CIP2A-TOPBP1 form large filamentous structures at sites of incomplete DNA replication during mitosis, and that CIP2A-TOPBP1 facilitate the recruitment of SMX tri-nuclease complex members SLX4, MUS81 and XPF-ERCC1. These structures form in proximity to sites of mitotic DNA synthesis, although CIP2A is not required for mitotic DNA synthesis. In addition to its globular and coiled-coil domain, the unstructured C-terminal domain of CIP2A is essential for CIP2A-TOPBP1 filamentous structure formation and recruitment of the SMX complex. BRCA1^-/- and BRCA2^-/- cells have increased mitotic DNA lesions that recruit CIP2A and SLX4. We show that the C-terminal part of CIP2A is required for survival of BRCA2^-/- cells. Moreover, SLX4 is crucial for genome stability in BRCA2^-/- cells. Combined, we demonstrate that CIP2A-TOPBP1 recruits the SMX complex during mitosis, which is required to resolve mitotic DNA lesions, allows faithful chromosome segregation and maintain viability of BRCA2^-/- cells.

< Back to publications

Full article >