2019
PloS one

Uptake of nanowires by human lung adenocarcinoma cells

Authors:

Abariute, L., Lard, M., Hebisch, E., & Prinz, C. N.

Keywords:

Nanowires, Silicon nanowires, Fluorescence microscopy, Cell membranes, Cell staining, Lysosomes, Adenocarcinoma of the lung, Reactive oxygen species

Abstract:

Semiconductor nanowires are increasingly used in optoelectronic devices. However, their effects on human health have not been assessed fully. Here, we investigate the effects of gallium phosphide nanowires on human lung adenocarcinoma cells. Four different geometries of nanowires were suspended in the cell culture for 48 hours. We show that cells internalize the nanowires and that the nanowires have no effect on cell proliferation rate, motility, viability and intracellular ROS levels. By blocking specific internalization pathways, we demonstrate that the nanowire uptake is the result of a combination of processes, requiring dynamin and actin polymerization, which suggests an internalization through macropinocytosis and phagocytosis.