abberior instruments
2021
Engineering
Fluorescence nanoscopy in neuroscience
Authors:
Wang, Y., Lin, J., Zhang, Q., Chen, X., Luan, H., & Gu, M.
Keywords:
Fluorescence imaging, Diffraction limit, Nanoscale resolution, Neuroscience
Abstract:
Fluorescence nanoscopy provides imaging techniques that overcome the diffraction-limited resolution barrier in light microscopy, thereby opening up a new area of research in biomedical imaging in fields such as neuroscience. Here, we review the foremost fluorescence nanoscopy techniques, including descriptions of their applications in elucidating protein architectures and mobility, the real-time determination of synaptic parameters involved in neural processes, three-dimensional imaging, and the tracking of nanoscale neural activity. We conclude by discussing the prospects of fluorescence nanoscopy, with a particular focus on its deployment in combination with related techniques (e.g., machine learning) in neuroscience.