abberior dyes & labels
Cell Reports Methods
Three-dimensional multi-color optical nanoscopy at sub-10-nm resolution based on small-molecule organic probes
Yubing Han, Shijie Tu, Wenwen Gong, Wenli Tao, Mingwei Tang, Yunfei Wei, Cuifang Kuang, Xu Liu, Yu-Hui Zhang, Xiang Hao
Imaging, super-resolution imaging, expansion microscopy, small-molecule organic probes, subcellular structures, interactions
Achieving nanometer-scale resolution remains challenging in expansion microscopy due to photon loss. To address this concern, here we develop a multi-color expansion stimulated emission depletion technique based on small-molecule probes to realize high labeling density and intensity. Our method substantially lowers the barrier to visualizing diverse intracellular proteins and their interactions in three dimensions. It enables us to achieve sub-10-nm resolution in structures such as microfilaments, lysosomes, and mitochondria, providing new insights into cell biology.