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MIRAVA POLYSCOPE – All in one and on for all: the perfect image
Science beyond Barriers

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Technology

2023
Cell Reports Methods

Three-dimensional multi-color optical nanoscopy at sub-10-nm resolution based on small-molecule organic probes

Authors:

Yubing Han, Shijie Tu, Wenwen Gong, Wenli Tao, Mingwei Tang, Yunfei Wei, Cuifang Kuang, Xu Liu, Yu-Hui Zhang, Xiang Hao

Keywords:

Imaging, super-resolution imaging, expansion microscopy, small-molecule organic probes, subcellular structures, interactions

Abstract:

Achieving nanometer-scale resolution remains challenging in expansion microscopy due to photon loss. To address this concern, here we develop a multi-color expansion stimulated emission depletion technique based on small-molecule probes to realize high labeling density and intensity. Our method substantially lowers the barrier to visualizing diverse intracellular proteins and their interactions in three dimensions. It enables us to achieve sub-10-nm resolution in structures such as microfilaments, lysosomes, and mitochondria, providing new insights into cell biology.

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Superresolution & Confocal Systems

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Superresolution & Confocal Modules

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  • MATRIX Detector
  • TIMEBOW Imaging
  • FLEXPOSURE Illumination
  • RAYSHAPE Mirror
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