2011
Nature methods

Sharper low-power STED nanoscopy by time gating

Authors:

Vicidomini, G., Moneron, G., Han, K. Y., Westphal, V., Ta, H., Reuss, M., ... & Hell, S. W.

Abstract:

Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.