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Science beyond Barriers

abberior intruments

Live Cell Imaging, Reviews

2021
Microscopy Today

See You at the Molecular Scale

Authors:

Rehman, J., Grimm, F., Schloetel, J. G., & Wurm, C. A.

Keywords:

live-cell imaging, organic live dyes, super-resolution STED microscopy, minimized light dose, MINFLUX microscopy

Abstract:

Live-cell fluorescence microscopy is an indispensable tool in biomedical and biophysical research, allowing the study of dynamic processes in living systems. Recent developments have given rise to high-end imaging systems capable of live-cell imaging on the nanometer scale. These instrumentation developments have, in turn, increased the demand for enhanced fluorophores to label target proteins within living cells. abberior offers solutions on both fronts. abberior LIVE dyes have higher cell permeability and improved specificity compared to conventional labels, making long-term live-cell imaging at extremely low probe concentrations possible. These low nanomolar working concentrations minimize cell toxic effects. abberior stimulation emission depletion (STED) microscopes offer super-resolution down to ~30 nm with minimal light exposure to the sample. This unique combination takes live-cell imaging to the next level and will make super-resolution imaging of dynamic subcellular processes a routine, easy-to-use visualization method, paving the way for fundamental biological discoveries. At the extreme, minimal emission fluxes (MINFLUX) microscopy offers molecular resolutions of a few nanometers in unprecedented temporal regimes.

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Superresolution & Confocal Systems

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