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Science beyond Barriers

abberior dyes & labels

2022
Analysis & Sensing

Rhodamine Fluorophores for STED Super‐Resolution Biological Imaging

Authors:

Chen, F., Liu, W., Li, H., Deng, T., Xing, B., & Liu, F

Abstract:

Among all fluorescent scaffolds, rhodamine and its derivatives have gained the greatest interest for super-resolution imaging based on stimulated emission depletion (STED) microscopy, because of their typically high brightness, excellent photo-stability and easy access to chemical derivatization. During the last few years, many successful examples have shown the imaging improvement by fine-tuning the chemical structure of rhodamine scaffolds. However, there is still a lack of overall summarization, particularly during the most recent five years. Herein, the recent achievements in the design of rhodamine dyes toward STED super-resolution bio-imaging are summarized, by classifying them into four parts according to different biological targets including the cytoskeletons, membranous organelles, DNA/RNAs and other functional substances. The basic principle for structure design as well as the relationships between chemical modification and the function for STED imaging will be mainly focused.

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