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Science beyond Barriers

abberior dyes & labels

Tailor-Made

2018
The Journal of organic chemistry

Photoactivatable rhodamine spiroamides and diazoketones decorated with “Universal Hydrophilizer” or hydroxyl groups

Authors:

Roubinet, B., Bischoff, M., Nizamov, S., Yan, S., Geisler, C., Stoldt, S., ... & Hell, S. W.

Keywords:

Dyes and pigments, Peptides and proteins, Fluorescence, Heterocyclic compounds, Organic compounds

Abstract:

Photoactivatable rhodamine spiroamides and spirocyclic diazoketones emerged recently as synthetic markers applicable in multicolor super-resolution microscopy. However, their applicability in single molecule localization microscopy (SMLM) is often limited by aggregation, unspecific adhesion, and low reactivity caused by insufficient solubility and precipitation from aqueous solutions. We report here two synthetic modifications increasing the polarity of compact polycyclic and hydrophobic labels decorated with a reactive group: attachment of 3-sulfo-l-alanyl-beta-alanine dipeptide (a “universal hydrophilizer”) or allylic hydroxylation in photosensitive rhodamine diazoketones (and spiroamides). The super-resolution images of tubulin and keratin filaments in fixed and living cells exemplify the performance of “blinking” spiroamides derived from N,N,N′,N′-tetramethyl rhodamine.

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