Imaging cellular ultrastructures using expansion microscopy (U-ExM)
Gambarotto, D., Zwettler, F. U., Le Guennec, M., Schmidt-Cernohorska, M., Fortun, D., Borgers, S., ... & Guichard, P.
Post-translational modifications, Super-resolution microscopy
The attribution of a protein to an ultrastructural element by optical microscopy represents a major challenge in biology. Here, we report a method of near-native expansion microscopy (U-ExM), enabling the visualization of preserved ultrastructures of macromolecules by optical microscopy. Combined with super-resolution, U-ExM unveiled the centriolar chirality, only visualizable by electron microscopy. We demonstrate the general applicability of U-ExM by imaging different cellular structures including microtubules and mitochondria in cellulo.