Imaging cellular ultrastructures using expansion microscopy (U-ExM)

Authors:

Gambarotto, D., Zwettler, F. U., Le Guennec, M., Schmidt-Cernohorska, M., Fortun, D., Borgers, S., ... & Guichard, P.

Keywords:

Post-translational modifications, Super-resolution microscopy

Abstract:

The attribution of a protein to an ultrastructural element by optical microscopy represents a major challenge in biology. Here, we report a method of near-native expansion microscopy (U-ExM), enabling the visualization of preserved ultrastructures of macromolecules by optical microscopy. Combined with super-resolution, U-ExM unveiled the centriolar chirality, only visualizable by electron microscopy. We demonstrate the general applicability of U-ExM by imaging different cellular structures including microtubules and mitochondria in cellulo.

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