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MIRAVA POLYSCOPE – All in one and on for all: the perfect image
Science beyond Barriers

abberior dyes & labels

Cell Biology, Neurobiology

2022
STAR protocols

Expansion microscopy of mouse brain organotypic slice cultures to study protein distribution

Authors:

Bissen, D., Kracht, M. K., Foss, F., & Acker-Palmer, A.

Keywords:

Cell Biology, Microscopy, Neuroscience

Abstract:

Assessing protein distribution with super-resolution in tissue is often complicated and restrictive. Here, we describe a protocol for immunostaining and expansion microscopy imaging of mouse brain organotypic slice cultures. We detail an Imaris analysis workflow to analyze the surface vs intracellular distribution of AMPA receptors at super-resolution during homeostatic plasticity. We have optimized the protocol for brain organotypic slice culture and tested in acute brain slices. This protocol is suitable to study protein distribution under multiple plasticity paradigms.

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Superresolution & Confocal Systems

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Superresolution & Confocal Modules

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  • MINFLUX Module
  • MATRIX Detector
  • TIMEBOW Imaging
  • FLEXPOSURE Illumination
  • RAYSHAPE Mirror
  • TRUESHARP Deconvolution
  • EASY3D
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