Expansion microscopy of mouse brain organotypic slice cultures to study protein distribution

Authors:

Bissen, D., Kracht, M. K., Foss, F., & Acker-Palmer, A.

Keywords:

Cell Biology, Microscopy, Neuroscience

Abstract:

Assessing protein distribution with super-resolution in tissue is often complicated and restrictive. Here, we describe a protocol for immunostaining and expansion microscopy imaging of mouse brain organotypic slice cultures. We detail an Imaris analysis workflow to analyze the surface vs intracellular distribution of AMPA receptors at super-resolution during homeostatic plasticity. We have optimized the protocol for brain organotypic slice culture and tested in acute brain slices. This protocol is suitable to study protein distribution under multiple plasticity paradigms.

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