abberior dyes & labels
2022
STAR protocols
Expansion microscopy of mouse brain organotypic slice cultures to study protein distribution
Authors:
Bissen, D., Kracht, M. K., Foss, F., & Acker-Palmer, A.
Keywords:
Cell Biology, Microscopy, Neuroscience
Abstract:
Assessing protein distribution with super-resolution in tissue is often complicated and restrictive. Here, we describe a protocol for immunostaining and expansion microscopy imaging of mouse brain organotypic slice cultures. We detail an Imaris analysis workflow to analyze the surface vs intracellular distribution of AMPA receptors at super-resolution during homeostatic plasticity. We have optimized the protocol for brain organotypic slice culture and tested in acute brain slices. This protocol is suitable to study protein distribution under multiple plasticity paradigms.